SGMS2 Antibody

文獻引用 (1) 說明書

中文名稱：

SGMS2兔多克隆抗體
貨號：

CSB-PA854159LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Rat Liver tissue lysate
All lanes: SGMS2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42/50 kDa
Observed band size: 42/50 kDa

Immunofluorescence staining of Hela cells with CSB-PA854159LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
引用文獻
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

The SGMS2 polyclonal antibody was produced by administering the recombinant human phosphatidylcholine: ceramide choline phosphotransferase 2 protein (1-79aa) to a rabbit to elicit an immune response. The resulting rabbit blood was collected and subjected to protein G affinity chromatography to purify the SGMS2 polyclonal antibody, which has a purity of over 95%.

This high-purity antibody has been shown to react with both human and rat samples and has been extensively validated for use in various applications, such as ELISA, WB, IF, and IHC. These applications allow for the detection of the presence and quantity of SGMS2 protein, visualization of its distribution and localization, and identification.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) SGMS2 Polyclonal antibody
Uniprot No.：

Q8NHU3
基因名：

SGMS2
別名：

SGMS2; SMS2; Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2
宿主：

Rabbit
反應(yīng)種屬：

Human, Rat
免疫原：

Recombinant Human Phosphatidylcholine:ceramide cholinephosphotransferase 2 protein (1-79AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，SGMS2 Antibody (CSB-PA854159LA01HU)，的標(biāo)記方式是Non-conjugated。對于SGMS2 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA854159LB01HU	SGMS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA854159LC01HU	SGMS2 Antibody, FITC conjugated
Biotin	CSB-PA854159LD01HU	SGMS2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

Expression of Ceramide-Metabolizing Enzymes in the Heart Adipose Tissue of Cardiovascular Disease Patients O Gruzdeva,International journal of molecular sciences,2023

產(chǎn)品評價

靶點詳情

功能：

Sphingomyelin synthase that primarily contributes to sphingomyelin synthesis and homeostasis at the plasma membrane. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis: in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction appears to depend on the levels of CER and DAG in the plasma membrane. Does not use free phosphorylcholine or CDP-choline as donors. Can also transfer phosphoethanolamine head group of phosphatidylethanolamine (PE) on to ceramide (CER) to form ceramide phosphoethanolamine (CPE). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting. To a lesser extent, plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1. Required for normal bone matrix mineralization.
基因功能參考文獻：
1. SMS regulates the expression and function of drug transporters P-gp and MRP2. PMID: 27394416
2. findings suggest that the C-terminal tails of SMSs are involved in homodimer formation, which is required for efficient transport from the ER. PMID: 27927984
3. PPARdelta activation may be a potential risk of atherosclerosis through enhancing activity of SMS2 PMID: 27278004
4. F-actin polymerization in the region of HIV-1 membrane fusion was more prominent in Sms2-expressing cells than Sms-deficient cells. PMID: 25231990
5. SMS1 and SMS2 are capable of regulating TGN-mediated protein trafficking and secretion PMID: 21980337
6. Data indicate that the increased sphingomyelin mass was due to a rapid and highly specific activation of sphingomyelin synthases SMS1 and SMS2. PMID: 22106271
7. direct morphological evidence for the pro-atherogenic capabilities of sphingomyelin synthase 2 PMID: 21235823
8. Data show that SMS2 acting as a bifunctional enzyme with both SM and CPE synthase activity. PMID: 19454763
9. Data show that sphingomyelin synthases SMS1 and SMS2 are co-expressed in a variety of cell types and function as the key Golgi- and plasma membrane-associated SM synthases in human cervical carcinoma HeLa cells, respectively. PMID: 17449912
10. Results indicate that both synthase (SMS)1 and 2 contribute to sphingomyelin (SM) de novo synthesis and control SM levels in the cells and on the cell membrane including plasma membrane. PMID: 17616479
11. SMS2 is a key factor in control of sphingomyelin and diacylglycerol metabolism within the cell, and thus it influences apoptosis. PMID: 17982138
12. SMS2 regulates subcellular pools of diacylglycerol-binding proteins in the Golgi apparatus. PMID: 18370930
13. SMS2 (Sphingomyelin synthase 2) physiologically contributes to de novo Sphingomyelin biosynthesis and plasma membrane Sphingomyelin levels PMID: 18566297
14. Both SMS1 and SMS2 contain two histidines and one aspartic acid which are conserved within the lipid phosphate phosphatase superfamily. Site-directed mutagenesis of these amino acids abolished SMS activity without altering cellular distribution. PMID: 18694848
15. These results suggested that posttranslational palmitoylation is important for determination of the subcellular localization of SMS2. PMID: 19233134
16. Sphingomyelin synthase 2 is one of the determinants for plasma and liver sphingomyelin levels in mice. PMID: 19286635
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein.
蛋白家族：

Sphingomyelin synthase family
組織特異性：

Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells.
數(shù)據(jù)庫鏈接：

HGNC: 28395

OMIM: 611574

KEGG: hsa:166929

STRING: 9606.ENSP00000351981

UniGene: Hs.595423

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