RASSF5 Antibody

說明書

中文名稱：

RASSF5兔多克隆抗體
貨號：

CSB-PA819906ESR1HU
規格：

￥440
圖片：
Western blot
All lanes: RASSF5antibody at 5.46μg/ml
Lane 1: Thp-1 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48, 31, 38 kDa
Observed band size: 48 kDa

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA819906ESR1HU at dilution of 1:100
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) RASSF5 Polyclonal antibody
Uniprot No.：

Q8WWW0
基因名：

RASSF5
別名：

Maxp 1 antibody; MAXP1 antibody; MGC10823 antibody; MGC17344 antibody; New ras effector 1 antibody; NORE 1 antibody; NORE1 antibody; NORE1A antibody; NORE1B antibody; Novel Ras effector 1 antibody; RAP1 Binding Protein antibody; RAPL antibody; Ras association (RalGDS/AF 6) domain family 5 antibody; Ras association (RalGDS/AF 6) domain family member 5 antibody; Ras association domain containing family protein 5 antibody; Ras association domain-containing protein 5 antibody; Ras effector like protein antibody; RASF5_HUMAN antibody; RASSF 3 antibody; RASSF 5 antibody; RASSF3 antibody; RASSF5 antibody; Regulator for cell adhesion and polarization enriched in lymphoid tissue antibody; Regulator for cell adhesion and polarization enriched in lymphoid tissues antibody; Tumor suppressor RASSF3 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Ras association domain-containing protein 5 protein (1-265AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing. May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.
基因功能參考文獻：
1. Ubc9 is an essential regulator of ADAP where it is required for TCR-induced membrane recruitment of the small GTPase Rap1 and its effector protein RapL. PMID: 29127148
2. Hepatitis C virus uses NS5B to specifically suppress NORE1A, facilitating viral replication and elevated Ras signaling. PMID: 28090674
3. Our study demonstrated that miR-214 expression was elevated and RASSF5 was down regulated in oral cancer. Moreover, miR-214 suppressed KB cell apoptosis through down regulation of RASSF5 expression PMID: 28290615
4. mCD40L-induced cell death mediated by NORE1A expression appeared to be independent of mCD40L-induced cell death mediated by sustained JNK activation since NORE1A inhibition did not affect JNK phosphorylation and vice versa PMID: 26986513
5. Ras induces the formation of a complex between NORE1A and the phosphatase PP1A, promoting the activation of the Rb tumor suppressor by dephosphorylation PMID: 26677227
6. Down-regulation of RASSF5A and RASSF5C expression is a tumor-specific phenomenon. PMID: 25420558
7. RASSF5 expression is negatively correlated with distant metastasis of osteosarcoma, and RASSF5 may function as a tumor suppressor in OS cells through activation of the MST1/LATS1 pathway. PMID: 25109282
8. NORE1A allows Ras to qualitatively modify p53 function to promote senescence. PMID: 25778922
9. the inactivation of RASSF5A through CpG island 1 methylation may play an important role in esophageal squamous cell carcinoma (ESCC) carcinogenesis, RASSF5A may be a functional tumor suppressor and may serve as a prognostic biomarker for ESCC. PMID: 25579665
10. NORE1A has a role in Ras regulation of SCF(beta-TrCP) protein activity and specificity PMID: 25217643
11. RASSF5 can act as an inhibitor or a potential positive regulator of Mst2, depending on whether it binds to Mst2 before or after activation-loop phosphorylation. PMID: 23972470
12. Ubiquitin ligase Itch is a unique negative regulator of RASSF5. PMID: 23538446
13. The RASSF gene family members RASSF5, RASSF6 and RASSF7 show frequent DNA methylation in neuroblastoma. PMID: 22695170
14. crystal of NORE1 diffracted to 2.7 A resolution and belonged to space group P6(1)22, with unit-cell parameters a = b = 73.041, c = 66.092 A, alpha = beta = 90, gamma = 120 degrees PMID: 22750872
15. These findings indicate that the control of HIPK1 stability by Mdm2-NORE1 has a major effect on cell behaviour, and epigenetic inactivation of NORE1 enables adenocarcinoma formation in vivo through HIPK1 stabilization. PMID: 22173032
16. NORE1A has activity that suppresses the centrosome amplification induced by HU and that NORE1A mRNA down-regulation is one of the common gene abnormalities in NSCLCs, both of which imply a key preventive role of NORE1A against the carcinogenesis of NSCLC. PMID: 20434789
17. epigenetic inactivation of NORE1 due to aberrant promoter hypermethylation is a frequent event in colorectal tumorigenesis and might be implicated in the malignant progression of colorectal tumors PMID: 20969767
18. a novel regulatory network composed of the tumor suppressor NORE1A, the mitotic kinase Aurora A, the small GTPase Ras, and the microtubule cytoskeleton. PMID: 20339001
19. findings define a T cell receptor "inside-out" pathway via N-SKAP1-C-RapL that regulates T cell adhesion, motility, and arrest times with dendritic cells in lymph nodes. PMID: 20346707
20. The present investigation provided evidence that Lck-mediated phosphorylation regulates the nucleocytoplasmic shuttling and cell growth control activities of RASSF5. PMID: 20064523
21. Nore1 is a member of a family of Ras effector/tumor suppressors that includes RASSF1 PMID: 12676952
22. the epigenetic alteration of NORE1A is confined to lung tumors with a wild-type K-ras: 88% (15 of 17) of the tumors with NORE1 hypermethylation did not harbor a K-ras mutation (P=0.008, Fisher's exact test) PMID: 15378027
23. Rap1 activation contributes to directional vascular endothelial cell migration accompanied by extension of microtubules on which RAPL localizes PMID: 15569673
24. results indicate that RASSF1A epigenetic changes are an early event in thyroid tumor pathogenesis and progression PMID: 15980887
25. Suppression of NORE1A, a known Ras effector, in PAX8-PPARgamma fusion-carrying follicular thyroid cancer. PMID: 16352687
26. association of NORE1A with cytoskeletal elements is essential for NORE1A-induced growth suppression and that the ERK pathway is a target for NORE1A growth-suppressive activities PMID: 16421102
27. The candidate tumor suppressor gene NORE1B is epigenetically down-regulated in hepatocellular carcinoma. PMID: 16516329
28. These findings suggest that endogenous Nore1B recruits active Ras to the APC-T cell interface and mediates the interaction between Ras and Carma1. PMID: 16520020
29. The NORE1A gene was never found epigenetically methylated in hepatitic or non-hepatitic liver. PMID: 16606445
30. Nuclear localization of RASSF5 is critical for its cell growth control activity. PMID: 17320110
31. interaction surfaces in RAPL-Rap1 and RAPL-Rap2 complexes are different and that a single residue in the switch I region of Rap proteins (residue 39) contributes considerably to the different kinetics of these protein-protein interactions. PMID: 17716979
32. nuclear export of NORE1A via nuclear export signal is involved in the NORE1A-mediated induction of apoptosis PMID: 18211824
33. NORE1A promoter methylation is a rare event in neuroblastoma cells and primary tumors. Other mechanisms are likely to account for the frequent reduction of NORE1A mRNA expression. Also, antitumorigenic role of NORE1A in human neuroblastoma is evident. PMID: 18452173
34. Study describes the crystal structure of Ras in complex with the Ras binding domain (RBD) of NORE1A; the contact area of NORE1A is extended as compared with other Ras effectors & provides a rationale for an exceptionally long lifetime of the complex. PMID: 18596699
35. findings showed that only 4% of the glioma tumors revealed a methylated promoter for NORE1A PMID: 18616639
36. Data show that degradation by calpains is a novel mechanism for downregulation of NORE1A and RASSF1A proteins and might be the mechanism allowing cancer cells to escape growth suppression. PMID: 19098985
37. NORE1B suppresses replication and transformation of cells as effectively as RASSF1A and is a putative tumor suppressor gene. NORE1B interacts with RASSF1A and loss of one of these may lead to uncontrolled growth and transformation of hepatocytes. PMID: 19118008
38. NORE1A activates p21(CIP1) via promoting p53 nuclear localization. PMID: 19435914
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亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A.
組織特異性：

Widely expressed. Frequently down-regulated in lung tumor cell lines and primary lung tumors.
數據庫鏈接：

HGNC: 17609

OMIM: 607020

KEGG: hsa:83593

STRING: 9606.ENSP00000347443

UniGene: Hs.497579

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