RAP1B Antibody

說明書

中文名稱：

RAP1B兔多克隆抗體
貨號：

CSB-PA841145
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA841145(RAP1B Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using CSB-PA841145(RAP1B Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 8%SDS-PAGE,Lysate: 40 μg,Lane 1-3: 293T cells, Jurkat cells, NIH/3T3 cells,Primary antibody: CSB-PA841145(RAP1B Antibody) at dilution 1/550 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P61224
基因名：

RAP1B
別名：

GTP-binding protein smg p21B antibody; K-REV antibody; OK/SW-cl.11 antibody; RAL1B antibody; rap1b antibody; RAP1B member of RAS oncogene family antibody; RAP1B_HUMAN antibody; Ras family small GTP binding protein RAP1B antibody; RAS related protein RAP1B antibody; Ras-related protein Rap-1b antibody; Small GTP binding protein antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Fusion protein of Human RAP1B
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Plays a role in the establishment of basal endothelial barrier function.
基因功能參考文獻：
1. Talin-F0 domain binds to Rap1b like canonical Rap1 effectors despite little sequence homology, and disruption of the binding strongly impairs integrin activation, cell adhesion, and cell spreading. PMID: 29170462
2. Aberrant expression of lncRNA AFAP1-AS, a competing endogenous RNA of miR-181a, may involve in the onset and progression of Hirschsprung disease by augmenting the miR-181a target gene, RAP1B. PMID: 28924375
3. In RCC tissues and renal carcinoma cells, miR-28-5p is expressed at a low level and RAP1B is expressed at a high level. miR-28-5p inhibits the tumorigenesis of RCC by directly downregulating RAP1B and influencing the activation of two MAP kinases in the MAPK signaling pathway, p38 and Erk1/2. PMID: 27729617
4. describe the methods for expression and purification of the plexin cytoplasmic region in E. coli, and characterization of its GAP activity using a photometric assay. We also provide a protocol for measuring GAP activity of single-chain constructs with Rap covalently linked to the plexin cytoplasmic region PMID: 27787845
5. Data show that RAP1B expression was up-regulated in esophageal squamous cell carcinoma (ESCC) clinical samples and RAP1B interacted with DVL2 and activated Wnt/beta-catenin signaling. PMID: 28119087
6. The protein expression of RAP1B and HIF-1 alpha contributed to gastric cancer malignant behavior and poor prognosis. PMID: 26329876
7. Cholera toxin and beta-adrenergic receptor activation phosphorylate Rap1B and inhibit its prenylation and membrane localization. PMID: 26209110
8. Glucocorticoids mediate induction of microRNA-708 to suppress ovarian cancer metastasis through targeting Rap1B. PMID: 25569036
9. Adenosine signaling reduces prenylation and plasma membrane localization of Rap1B, resulting in enhanced tumor cell scattering and invasiveness. PMID: 23716715
10. High concentrations of extracellular adenosine in the tumor microenvironment can chronically activate A2B receptors to suppress Rap1B prenylation and signaling at the cell membrane, resulting in reduced cell-cell contact and promoting cell scattering. PMID: 23716716
11. TMZ as a standard chemotherapeutic agent for GBM inhibits the Rap1B expression and actin cytoskeleton remodeling to exert its cell killing by upregulating miR-181a/b/c/d subunits PMID: 24573637
12. Rap1b in both smooth muscle and endothelium plays a key role in maintaining blood pressure by controlling normal vascular tone. PMID: 24790136
13. findings show Rap1b expression decreased significantly in tubules of renal biopsies from patients with diabetic nephropathy (DN); findings indicate Rap1b ameliorates tubular injury and slows progression of DN by modulation of mitochondrial dysfunction via C/EBP-beta-PGC-1alpha signaling PMID: 24353183
14. Hepatitis B virus down-regulated miR-101-3p expression, which resulted in up-regulation of Rap1b, and down-regulation of miR-101-3p or up-regulation of Rap1b promoted proliferation and migration of hepatocellular carcinoma cells PMID: 24697700
15. phosphorylation of CalDAG-GEFI is a critical mechanism by which PKA controls Rap1b-dependent platelet aggregation PMID: 23611601
16. miR-139 decreases proliferation by directly targeting RAP1B PMID: 22642900
17. miR-518b was down-regulated in esophageal squamous cell carcinoma tissues and correlated with metastasis and survival. Furthermore, Rap1b was revealed to be directly regulated by miR-518b PMID: 22958893
18. Relationships between Rap1b, affinity modulation of integrin alpha IIbbeta 3, and the actin cytoskeleton (integrin alpha(IIb)beta(3)) PMID: 11994301
19. Data show that stimulation of a G(i)-dependent signaling pathway causes activation of Rap1B through the action of the PI3K product PtdIns(3,4,5)P(3) and that this process may contribute to potentiation of platelet aggregation. PMID: 12407113
20. RAP1B is prominently expressed in the nucleus of squamous carcinomas. PMID: 13679863
21. PAR-1 and PAR-4 signal Rap1B activation, the ability of thrombin to activate this GTPase independently of secreted ADP involves costimulation of both receptors as well as binding to GPIb-IX-V. PMID: 15078882
22. TPO integrates G(i), but not G(q), stimulation, supports integrin alpha(IIb)beta(3) activation platelet aggregation independently of phospholipase C but requires PI3-kinase and Rap1B PMID: 15863506
23. The RAP1B gene mutation is a rare event in myelodysplastic syndromes(MDS) bone marrow cells, in detecting RAP1B mutations, random mutagenesis was performed in RAP1B exons 2 and 3. PMID: 16118622
24. Rap1b is an important element in integrin-dependent outside-in signaling during platelet adhesion and regulates the cross talk between adhesive receptors. PMID: 16357324
25. These results demonstrate that Rap2b, but not the more abundant Rap1b, is associated to lipid rafts in human platelets. PMID: 18582561
26. structure of Epac2 in complex with a cAMP analogue (Sp-cAMPS) and RAP1B by X-ray crystallography and single particle electron microscopy PMID: 18660803
27. Inhibition of PI3K or mTOR reduced the level of Rap1B, which acts downstream of Rheb and mTOR. The ubiquitin E3 ligase Smurf2 mediates the restriction of Rap1B by initiating its degradation. PMID: 18842593
28. a mechanistic explanation for the differential effects of Rap1 phosphorylation by PKA on effector protein interaction PMID: 19651783
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亞細胞定位：

Cell membrane. Cytoplasm, cytosol. Cell junction. Note=May shuttle between plasma membrane and cytosol. Presence of KRIT1 and CDH5 is required for its localization to the cell junction.
蛋白家族：

Small GTPase superfamily, Ras family
數據庫鏈接：

HGNC: 9857

OMIM: 179530

KEGG: hsa:5908

STRING: 9606.ENSP00000250559

UniGene: Hs.369920

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