RAD17 Antibody

中文名稱：

RAD17兔多克隆抗體
貨號：

CSB-PA019253EA01HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: RAD17 antibody at 12µg/ml + Mouse thymus tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 78, 76, 58, 67 kDa
Observed band size: 78 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA019253EA01HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) RAD17 Polyclonal antibody
Uniprot No.：

O75943
基因名：

RAD17
別名：

CCYC antibody; Cell cycle checkpoint protein antibody; Cell cycle checkpoint protein RAD17 antibody; FLJ41520 antibody; HRAD 17 antibody; hRad17 antibody; R24L antibody; Rad 17 antibody; Rad 24 antibody; RAD1 (S. pombe) homolog antibody; RAD1 homolog antibody; RAD17 antibody; RAD17 homolog (S. pombe) antibody; RAD17 homolog antibody; Rad17 like protein antibody; RAD17; S. pombe; homolog of antibody; RAD17_HUMAN antibody; RAD17Sp antibody; Rad24 antibody; Rad24; mouse; homolog of antibody; Rad24; S. cerevisiae; homolog of antibody; RF C activator 1 homolog antibody; RF C/activator 1 homolog antibody; RF-C/activator 1 homolog antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Cell cycle checkpoint protein RAD17 protein (1-270AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，RAD17 Antibody (CSB-PA019253EA01HU)，的標(biāo)記方式是Non-conjugated。對于RAD17 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA019253EB01HU	RAD17 Antibody, HRP conjugated	ELISA
FITC	CSB-PA019253EC01HU	RAD17 Antibody, FITC conjugated
Biotin	CSB-PA019253ED01HU	RAD17 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
基因功能參考文獻：
1. DDX11 orchestrates jointly with 9-1-1 and its loader, RAD17, DNA damage tolerance at sites of bulky lesions, and endogenous abasic sites. These functions may explain the essential roles of DDX11 and its similarity with 9-1-1 during development. PMID: 30061412
2. The Rad17 C-terminal tail is a molecular switch that regulates the 9-1-1 interaction and the ATR pathway. PMID: 28666868
3. These data indicate that the interaction with the 9-1-1 complex is not required for Rad17 protein to be an efficient substrate for the UV-induced phosphorylation. Our data also raise the possibility that the 9-1-1 complex plays a negative regulatory role in the Rad17 phosphorylation. We also show that the nucleotide-binding activity of Rad17 is required for its nuclear localization. PMID: 27387238
4. In a Japanese population, the variant allele of hRAD17 is significantly associated with a decreased risk of Colorectal Cancer among light smokers and rectal cancer patients and with an increased risk of Colorectal Cancer among heavy smokers. PMID: 28238011
5. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
6. USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure. We show that USP20 regulation of Rad17 is at the protein level in a proteasome-dependent manner. USP20 depletion results in poor activation of Chk1 protein by phosphorylation PMID: 24923443
7. Rad17 is phosphorylated by ATM at Thr622 resulting in a direct interaction of Rad17 with NBS1, facilitating recruitment of MRE11, RAD50 and ATM to the DNA double-strand breaks. PMID: 24534091
8. Our data suggest RAD17 as a novel target protein for gemcitabine combination therapy supplementing or complementing inhibition of CHK1. PMID: 23687379
9. Knockdown of Rad17 with two independent siRNAs significantly reduced Chk1 phosphorylation and substantial RPA32 Ser33 phosphorylation. PMID: 23684611
10. Data indicate that regulation of Rad17 turnover is through the Cdh1/anaphase-promoting complex pathway in breast cancer cells. PMID: 23637229
11. Proteolysis of Rad17 by Cdh1/APC regulates checkpoint termination and recovery from genotoxic stress PMID: 20424596
12. The four alternatively spliced forms differentially expressed in different tissues, in different phases of the cell cycle, and differentially responded to X-irradiation. PMID: 11602352
13. upon replication block a Rad17/RF-C complex is recruited to sites of DNA lesions in late S phase, binds the Rad9/Hus1/Rad1 complex and enables it to interact with PCNA. An interaction of Rad17/RF-C with PCNA is mediated by the small RF-C p37 subunit PMID: 12400013
14. Rad17 localizes to DNA replication sites and interacts with DNA polymerase epsilon. PMID: 14500819
15. replication protein A (RPA) stimulates the binding of the Rad17-Rfc2-5 complex to single-stranded DNA PMID: 14605214
16. we show a requirement for Rad17 and Hus1 to induce G(2) arrest as well as Vpr-induced phosphorylation of histone 2A variant X (H2AX) and formation of nuclear foci containing H2AX and breast cancer susceptibility protein 1 PMID: 15485898
17. interacts with newly identified hMCM7 protein, a core component of the DNA replication apparatus PMID: 15538388
18. Findings reveal a phosphorylation-dependent function of Rad17 in an ATR-Rad17-Claspin-Chk1-signaling cascade that responds to specific replication stress. PMID: 16885023
19. Using siRNA to knock down Rad17 demonstrates that it is not essntial for the DNA replication checkpoint in Hela cells. PMID: 16951182
20. Loss of hRAD17 expression occurs frequently in HNSCC, is often due to genomic deletion, and may facilitate genomic instability in HNSCC PMID: 17657792
21. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. PMID: 18378394
顯示更多

收起更多
亞細胞定位：

Nucleus. Note=Phosphorylated form redistributes to discrete nuclear foci upon DNA damage.
蛋白家族：

Rad17/RAD24 family
組織特異性：

Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expr
數(shù)據(jù)庫鏈接：

HGNC: 9807

OMIM: 603139

KEGG: hsa:5884

STRING: 9606.ENSP00000370151

UniGene: Hs.16184

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
VIM Recombinant Monoclonal Antibody

Applications: ELISA, IHC, IF

Species Reactivity: Human