RAD1 Antibody

說明書

中文名稱：

RAD1兔多克隆抗體
貨號：

CSB-PA019252ESR1HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: Cell cycle checkpoint protein RAD1 antibody at 6μg/ml + NIH/3T3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 32, 28, 8 kDa
Observed band size: 32 kDa

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA019252ESR1HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) RAD1 Polyclonal antibody
Uniprot No.：

O60671
基因名：

RAD1
別名：

Cell cycle checkpoint protein Hrad1 antibody; Cell cycle checkpoint protein Rad 1 A / B antibody; Cell cycle checkpoint protein RAD1 antibody; Checkpoint control protein HRAD1 antibody; Checkpoint control protein RAD1 antibody; DNA repair exonuclease antibody; DNA repair exonuclease rad1 antibody; DNA repair exonuclease rad1 homolog antibody; DNA repair exonuclease REC1 antibody; DNA repair protein RAD1 antibody; EC 3.1.11.2 antibody; Exonuclease homolog RAD1 antibody; GTP-binding protein RAD antibody; hRAD 1 antibody; hRAD1 antibody; MGC77779 antibody; RAD 1 antibody; RAD antibody; RAD1 antibody; RAD1 homolog (S. pombe) antibody; RAD1 homolog antibody; Rad1 like DNA damage checkpoint antibody; Rad1 like DNA damage checkpoint protein antibody; RAD1; S. pombe; homolog of antibody; Rad1-like DNA damage checkpoint protein antibody; RAD1_HUMAN antibody; Ras associated with diabetes antibody; REC 1 antibody; REC1 antibody; RRAD antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Cell cycle checkpoint protein RAD1 protein (1-282AA)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:200-1:1000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
基因功能參考文獻(xiàn)：
1. Dominant alleles of RAD51, TP53 and XRCC1 combined genotypes indicated a strong protective role against hereditary breast cancer. PMID: 26954070
2. Intramolecular binding of the rad9 C-terminus in the checkpoint clamp Rad9-Hus1-Rad1 is closely linked with its DNA binding. PMID: 26088138
3. Data show models for the ternary PCNA/FEN1/DNA and Rad9-Rad1-Hus1 (9-1-1 complex)/FEN1/DNA assemblies. PMID: 22586102
4. The RAD1 is loaded to damaged sites where it serves as a platform for the selective recruitment of checkpoint and repair proteins. PMID: 21978893
5. CK2 plays a crucial role in the ATR-dependent checkpoint pathway through its ability to phosphorylate Ser-341 and Ser-387 of the Rad9 subunit of the Rad9-Hus1-Rad1 complex PMID: 20545769
6. 9-1-1 complex is a component of the mismatch repair involved in MNNG-induced damage response. PMID: 20188637
7. Rad9-Rad1-Hus1 complex enhances in vitro activity of 8-oxoguanine DNA glycosylase. PMID: 19615952
8. Rad9, Hus1, and Rad1 heterotrimeric complex chromatin binding is a proximal event in the checkpoint signaling cascade PMID: 12228248
9. RAD1 is a potential intrinsic chaperone in the stabilization of HUS1 for the heterotrimeric (RAD9-RAD1-HUS1) checkpoint complex formation. PMID: 15122316
10. The human Rad9/Rad1/Hus1 complex interacts with and stimulates DNA polymerase beta activity. PMID: 15314187
11. complex with rad9 and hus1 is a damage-specific activator of flap endonuclease 1 PMID: 15556996
12. The long-patch base excision machinery is an important target of the Rad9-Rad1-Hus1 complex, thus enhancing the quality control of DNA. PMID: 15871698
13. PCNA and the Rad9/Rad1/Hus1 complex can independently bind and activate Fen1; acetylation of Fen1 by p300-HAT abolished the stimulatory effect of the complex but not that of PCNA, suggesting a possible mechanism of regulation of this repair pathway PMID: 16216273
14. human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
15. These data provide in vivo evidence that the human 9-1-1 complex participates in DNA repair in addition to its previously described role in DNA damage sensing. PMID: 16814252
16. Human NEIL1 DNA glycosylase activity is significantly stimulated by hRad1 and by the Rad9/Rad1/Hus1 heterotrimer. PMID: 17395641
17. we report successful tri-cistronic cloning, overexpression and purification of a three-protein complex of Rad9, Rad1 and Hus1 using a single hexa-histidine tag. PMID: 17493829
18. Jab1 physically associates with the 9-1-1 complex; this association is mediated through direct interaction between Jab1 and Rad1, one of the subunits of the 9-1-1 complex PMID: 17583730
19. Human thymine DNA glycosylase activity is significantly stimulated by hHus1, hRad1, hRad9 separately, and by the 9-1-1 complex. PMID: 17855402
20. The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
21. The interdomain connecting loops (IDC loop) of hRad9, hHus1, and hRad1 are largely divergent and unique structural features of the 9-1-1 complex that are proposed to contribute to DNA damage recognition. PMID: 19535328
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

Rad1 family
組織特異性：

Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
數(shù)據(jù)庫鏈接：

HGNC: 9806

OMIM: 603153

KEGG: hsa:5810

STRING: 9606.ENSP00000340879

UniGene: Hs.38114

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