Phospho-TOB1 (Ser164) Antibody

說明書

中文名稱：

磷酸化-TOB1 (Ser164)兔多克隆抗體
貨號：

CSB-PA957092
規格：

￥2454
圖片：
Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody. The lane on the left is treated with antigen-specific peptide.

Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody (left)or the same antibody preincubated with blocking peptide (right).
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TOB1 Polyclonal antibody
Uniprot No.：

P50616
基因名：

TOB1
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Mediates CPEB3-accelerated mRNA deadenylation by binding to CPEB3 and recruiting CNOT7 which leads to target mRNA deadenylation and decay.
基因功能參考文獻：
1. MiR-590 targets Tob1 that is significantly decreased in patients with multiple sclerosis. PMID: 28947212
2. The dual-luciferase reporter assay also confirmed that miR-25 harbored the A allele which caused an incapacitation of binding at the TOB1. In conclusion, rs41274221 in miR-25 was a subgroup which may protect the patients from further growth and metastasis of gastric cancer and might serve as a novel biomarker for the disease PMID: 26572149
3. A novel role for TOB1 in mediating survival of estrogen-independent breast cancers. PMID: 26165839
4. Reduced TOB1 expression in gastric adenocarcinoma is associated with the extent of differentiation and the TNM stage of gastric cancer. PMID: 25760308
5. miR-25 promotes GC progression by directly downregulating TOB1 expression, and may be a noninvasive biomarker for the prognosis of GC patients. PMID: 25043310
6. arsenite-induced oxidative stress inhibits deadenylation of mRNA primarily through downregulation of Tob and Pan3, both of which mediate the recruitment of deadenylases to mRNA PMID: 25446091
7. Tob is a key factor in the regulation of c-myc gene expression, which is essential for cell growth. PMID: 23178487
8. TOB1 demonstrated a radioprotective function in the immortalized normal human bronchial epithelial cell line. PMID: 23756562
9. TOB1 modulates the radiosensitivity of lung cancer cells via the MAPK/ERK signaling pathway. PMID: 23589165
10. Cdc7 phosphorylates and interacts with Tob to inhibit the Cul4-DDB1(Cdt2)-dependent Tob degradation. PMID: 23066029
11. study demonstrates that the overexpression of Tob1 inhibits gastric cancer progression by activating Smad4- and inhibiting betacatenin-mediated signaling pathways PMID: 22710759
12. TOB1 overexpression not only increased the expression of the phosphatase and tensin homolog (PTEN), an important tumor suppressor, but also regulated the downstream effectors in the PI3K/PTEN signaling pathway, including Akt, ERK1/2, etc. PMID: 22158108
13. The antiproliferative and the mRNA deadenylation/decay-promoting effects of TOB1 and TOB2 proteins are linked. PMID: 22252318
14. Silencing of HIC1 and TOB1 expression is a common occurrence in gastric cancer and may contribute to the development and progression of the disease. PMID: 21533545
15. Longer survival was associated with hypomethylation at specific CpG sites (e.g. GREB1, TGIF and TOB1) and hypermethylation in other genes (e.g. TMCO5, PTPRN and GUCY2C). PMID: 21577013
16. Together, these results indicate that Tob mediates the recruitment of Caf1 to the target of CPEB3 and elicits deadenylation and decay of the mRNA. PMID: 21336257
17. Tob interactions with Caf1 and a C-terminal domain of PABPC1 PMID: 20595394
18. functions as lateral signal transmitter PMID: 11904957
19. Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system. the C-terminal regions are necessary and sufficient to control the stabilities of BTG1, BTG2, Tob, and Tob2 proteins. PMID: 12135500
20. identified as a MAPK substrate PMID: 12151396
21. Decreased expression or phosphorylation status of tob protein is associated with lung cancer PMID: 14643028
22. N-terminal region of TOB is a functional nuclear export signal. PMID: 15051490
23. Tob is involved in the translational suppression of IL-2 mRNA in anergic T cells through its interaction with iPABP. PMID: 15676026
24. Tob1 is a novel target for degradation by the SCF-Skp2 ubiquitin ligase in a cell line. PMID: 16951159
25. Reverse-transcription polymerase chain reaction confirmed increased expression of GADD45A, BTG2, PDE4B, and CEBPD and downregulation of TOB1 in skeletal muscle intradialysis. PMID: 16997058
26. Subtractive hybridization identified Twisted gastrulation (Tsg) as one of the genes suppressed by Tob. PMID: 17164348
27. Data show that TOB enhances mRNA deadenylation in vivo, and that interaction with PABPC1 is necessary for TOB's deadenylation-enhancing effect. PMID: 17785442
28. antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized PMID: 18084094
29. Tob associates with the CCR4-NOT complex. PMID: 18377426
30. positive correlation of TOB1 phosphorylation status with proliferation marker Ki67 suggests that elevated TOB1 phosphorylation might abrogate the antiproliferative effect of TOB1 in breast cancer PMID: 19491269
31. Down-regulation of TOB is associated with breast cancer tumorigenesis. PMID: 19569230
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

BTG family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 11979

OMIM: 605523

KEGG: hsa:10140

STRING: 9606.ENSP00000268957

UniGene: Hs.703321

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