Phospho-HIST1H1D (T179) Antibody
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中文名稱：磷酸化-HIST1H1D (T179)兔多克隆抗體
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貨號：CSB-PA010379OA179phHU
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規格：￥660
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圖片：
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Immunofluorescence staining of Hela cells with CSB-PA010379OA179phHU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-HIST1H1D (CSB-PA010379OA179phHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) HIST1H1D Polyclonal antibody
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Uniprot No.：
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基因名：HIST1H1D
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別名：H1 histone family member 3 antibody; H1.3 antibody; H13_HUMAN antibody; H1F3 antibody; HIST1 H1D antibody; HIST1H1D antibody; Histone 1 H1d antibody; Histone cluster 1 H1d antibody; Histone H1.3 antibody; Histone H1c antibody; MGC138176 antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Peptide sequence around site of Phospho-Thr (179) derived from Human Histone H1.3
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, IF, ChIP
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推薦稀釋比：
Application Recommended Dilution IF 1:20-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
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靶點詳情
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功能：Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
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基因功能參考文獻：
- Results show that histone H1.3, was identified only in non-neoplastic MCF-10A breast cells but not in metastatic MDA-MB-231 breast cancer cells . PMID: 26209608
- Histone H1 organizes and maintains an extensive protein-protein interaction network in the nucleolus required for nucleolar structure and integrity. PMID: 25584861
- overexpression of histone cluster 1 is associated with recurrence in meningiomas. PMID: 20685720
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亞細胞定位：Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin.
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蛋白家族：Histone H1/H5 family
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數據庫鏈接：
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