Phospho-DAPK2 (S318) Antibody

說明書

中文名稱：

磷酸化-DAPK2 (S318)兔多克隆抗體
貨號：

CSB-PA008412
規格：

￥1090
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q9UIK4
基因名：

DAPK2
別名：

DAK2 antibody; DAP kinase 2 antibody; DAP kinase related protein 1 antibody; DAP kinase related protein 1 beta isoform antibody; DAP-kinase-related protein 1 antibody; DAPK2 antibody; DAPK2_HUMAN antibody; Death associated protein kinase 2 antibody; Death-associated protein kinase 2 antibody; DRP 1 antibody; DRP-1 antibody; DRP1 antibody; MGC119312 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human DAPK2 around the phosphorylation site of S318.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

IHC, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Protocols：

Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Regulates granulocytic motility by controlling cell spreading and polarization.; Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.
基因功能參考文獻：
1. study reveals a unique calmodulin-independent mechanism for DAPK2 activation, critical to its function as a novel downstream effector of AMPK in autophagy PMID: 29717115
2. This study suggests that miR-520g contributes to tumor progression and drug resistance by post-transcriptionally downregulating DAPK2 in patients with epithelial ovarian cancer PMID: 27049921
3. Thyroid hormone promotes selective autophagy via induction of DAPK2-SQSTM1 cascade, which in turn protects hepatocytes from diethylnitrosamine-induced hepatotoxicity or carcinogenesis. PMID: 27653365
4. miR-520h suppresses Death-associated protein kinase 2 (DAPK2) expression, as restoring DAPK2 abolished miR-520h-promoted drug resistance, and knockdown of DAPK2 mitigated cell death caused by the depletion of miR-520h. PMID: 25982274
5. that Death-associated protein kinase 2 effector functions are influenced by the protein's subcellular localization PMID: 26483415
6. This study links adipocyte expression of an autophagy-regulating kinase, lysosome-mediated clearance and fat cell lipid accumulation; it demonstrates obesity-related attenuated autophagy in adipocytes, and identifies DAPK2 dependence in this regulation. PMID: 26038578
7. DAPK2 is a novel kinase of mTORC1 and is a potential new member of this multiprotein complex, modulating mTORC1 activity and autophagy levels under stress and steady-state conditions. PMID: 25361081
8. DAPK2 regulates oxidative stress in cancer cells by preserving mitochondrial function PMID: 25741596
9. DAPK2-induced apoptosis is negatively regulated by Akt and 14-3-3 proteins. PMID: 26047703
10. DAPK2 is upregulated in uterosacral ligaments in pelvic organ prolapse PMID: 23700042
11. The defect in chemotaxis in DAPK2-inactive granulocytes is likely a result of reduced polarization of the cells, mediated by a lack of MLC phosphorylation, resulting in radial F-actin and pseudopod formation. PMID: 24163421
12. The tumor suppressor gene DAPK2 is induced by the myeloid transcription factors PU.1 and C/EBPalpha during granulocytic differentiation but repressed by PML-RARalpha in APL. PMID: 24038216
13. DRP-1 and ZIPk most likely evolved from their ancient ancestor gene DAPk by two gene duplication events that occurred close to the emergence of vertebrates PMID: 21408167
14. Sodium butyrate induced DAPK1/2 expression in human gastric cancer cells and this expression prompted apoptosis by decreasing FAK levels. PMID: 22160140
15. High frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients PMID: 11839660
16. distinct methylation pattern in bladder cancer with frequent methylation of RARbeta, DAPK, E-cadherin, and p16. PMID: 11839665
17. gene expression in colorectal and gastric cancer silenced by DNA methylation and histone deacetylation PMID: 12087472
18. Results implicate a novel role for DAPK2 in the regulation of normal myelopoiesis. PMID: 17347302
19. DAPK2 as a novel Sp1-dependent target gene for E2F1 and KLF6 in cell death response. PMID: 18521079
20. beta-catenin-induced down-regulation of DAPk-2 represents a novel signaling mechanism by which beta-catenin promotes the survival of malignant epithelial cells PMID: 18957423
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亞細胞定位：

Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, DAP kinase subfamily
組織特異性：

Expressed in neutrophils and eosinophils. Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
數據庫鏈接：

HGNC: 2675

OMIM: 616567

KEGG: hsa:23604

STRING: 9606.ENSP00000261891

UniGene: Hs.237886

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