Phospho-BIK (T33) Antibody

說明書

中文名稱：

磷酸化-BIK (T33)兔多克隆抗體
貨號：

CSB-PA040303
規格：

￥1090
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q13323
基因名：

BIK
別名：

BIK; NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4
宿主：

Rabbit
反應種屬：

Human
免疫原：

Synthesized peptide derived from Human NBK around the phosphorylation site of T33.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:10000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
基因功能參考文獻：
1. the results revealed the autophagy modulator TMEM74 interrelates with apoptosis inducer BIK and inhibits its function. PMID: 28412412
2. Data suggest that the ERalpha-H19-BIK signaling axis plays an important role in promoting breast cancer cell chemoresistance. PMID: 27845892
3. Suggest complex mechanism of tumor promotion in Bik high breast tumors. PMID: 27120789
4. BIK significantly contributes to DNA damage-induced mitochondrial apoptosis in HCT-116 wt cells upstream of the second peak of ROS production, BAX and BAK activation, cytochrome c release and caspase activation. PMID: 28796811
5. These studies suggest a link between Bik-mediated caspase activation and cleavage of viral proteins. PMID: 26437021
6. our data demonstrated that suppression of BIK in ER-positive MCF-7 cells prevents the cytotoxic effect of TAM and favors a more aggressive phenotype, due to the molecular change of different pathways PMID: 25861752
7. HCV RNA replication and release were significantly suppressed in BIK-depleted cells and over-expression of the RNA-dependent RNA polymerase, NS5B, was able to induce BIK expression PMID: 25463603
8. BikDDA, a novel mutant of Bik, showed a prolonged half-life and enhanced pro-apoptotic ability in triple-negative breast cancer cells compared with BikDD. PMID: 24637719
9. Findings identify novel cross talk between autophagy and apoptosis, wherein targeting SQSTM1/p62 converts cytoprotective autophagy to an inefficient form due to cargo loading failure, leading to NBK/Bik accumulation, which triggers apoptosis. PMID: 25002530
10. Authors show that human herpesvirus 4 EBNA2 represses BIK in B-cell lymphoma-derived cell lines and that this host-virus interaction can inhibit the proapoptotic effect of transforming growth factor beta1. PMID: 24554662
11. Data suggest BIK expression in tumor cells is not subject to direct regulation by MAP kinase signaling; BIK expression appears to be cell-cycle-dependent and increases in G1 cell-cycle arrest which results from inhibition of MAP kinase signaling. PMID: 24527759
12. BIK/NBK gene expression may have important clinical implications and provide predictive, prognostic or therapeutic marker in breast cancer patients PMID: 22855140
13. Src tyrosine kinase inhibits apoptosis through the Erk1/2- dependent degradation of the death accelerator Bik. PMID: 22388352
14. A previously undescribed indirect epigenetic regulation of BIK in FA-C lymphoblasts is mediated by DeltaNp73, an isoform of p73 lacking its transactivation domain that activates BIK through a proximal element in its promoter. PMID: 22873408
15. Data indicate that methylation-induced transcriptional silencing of the BIK (bcl2-interacting killer) pro-apoptotic gene may occur in multiple myeloma (MM), which might serve as a predictor of the development of relapsed/refractory MM. PMID: 22288719
16. Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK. PMID: 22139380
17. Bik has a role in both, apoptosis induction and sensitivity to oxidative stress in myeloma cells. PMID: 21063407
18. Systemic tumor suppression by the proapoptotic gene bik. PMID: 11782349
19. The results identify BIK as an initiator of cytochrome c release from mitochondria operating from a location at the ER. PMID: 11884414
20. NBK mediates apoptosis entirely by BAX-dependent mitochondrial pathway. PMID: 12853473
21. Several sequence alterations of the BIK gene have been identified in peripheral B-cell lymphomas which may have a role in disease pathogenesis. PMID: 12874789
22. Bik is induced in MCF-7 cells in the absence of estrogen signaling and plays a critical role in the antiestrogen-provoked breast cancer cell apoptosis. PMID: 14983013
23. degraded in Chlamydia trachomatis-infected cells. PMID: 15731089
24. Bik and Bim have roles in bortezomib sensitization of cells to killing by death receptor ligand TRAIL PMID: 15767553
25. Data show that BIK activates recruitment of DRP1 to the surface of the endoplasmic reticulum in intact cells, resulting in mitochondrial fragmentation but little release of cytochrome c to the cytosol. PMID: 15791210
26. Endogenous cellular BIK, therefore, regulates a BAX,BAK-dependent ER pathway that contributes to mitochondrial apoptosis PMID: 15809295
27. Bik/NBK accumulation was caused by stabilization of the protein from degradation and was associated with bortezomib cytotoxicity and apoptosis induction. PMID: 15824729
28. Bik does not have a definitive role in development and progression of sporadic breast neoplasms in Mexican females PMID: 16060964
29. E2Fs transactivate bik by a p53-independent mechanism. PMID: 17027756
30. Results suggest that expression of BIK in human breast cancer cells is regulated at the mRNA level by a mechanism involving a nontranscriptional activity of p53 and by proteasomal degradation of BIK protein. PMID: 17047080
31. The activation of caspase-9 and depolarization of mitochondrial membrane potential were induced by BIK, which were decreased concomitant with caspase-12 silenced. PMID: 17574210
32. Genes encoding KU70, MGST1 and BIK show age-related mRNA expression levels in hematopoietic stem cells. PMID: 17714764
33. The depletion of ER Ca2+ stores rather than the elevation of cytosolic Ca2+ or the extracellular Ca2+ entry contributed to Bik-induced Hep3B cells apoptosis. PMID: 18299962
34. BIK might not play a major role in the susceptibility of schizophrenia in Japanese population. PMID: 19632297
35. BIK is mainly localized in the ER & induces apoptosis through the mitochondrial pathway. It is involved in mature B cell selection. It a pro-apoptotic tumor suppressor in several human tissues. Review. PMID: 19641504
36. Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826
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亞細胞定位：

Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes.
數據庫鏈接：

HGNC: 1051

OMIM: 603392

KEGG: hsa:638

STRING: 9606.ENSP00000216115

UniGene: Hs.475055

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