Phospho-ATRIP (S68) Antibody

說明書

中文名稱：

磷酸化-ATRIP (S68)兔多克隆抗體
貨號：

CSB-PA060304
規格：

￥1090
圖片：
Western Blot analysis of NIH-3T3 cells using Phospho-ATRIP (S68) Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q8WXE1
基因名：

ATRIP
別名：

AGS1 antibody; ATIP antibody; ATM and Rad3 related interacting protein antibody; ATM and Rad3-related-interacting protein antibody; ATR interacting protein antibody; ATR-interacting protein antibody; Atrip antibody; ATRIP_HUMAN antibody; DKFZp762J2115 antibody; FLJ12343 antibody; MGC20625 antibody; MGC21482 antibody; MGC26740 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human ATRIP around the phosphorylation site of S68.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:40000

Protocols：

Western Blotting (WB) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
基因功能參考文獻：
1. ATRIP deacetylation by SIRT2 promotes ATR-ATRIP binding to replication protein A-single-stranded DNA to drive ATR activation and thus facilitate recovery from replication stress. PMID: 26854234
2. ATRIP SUMOylation promotes ATR activation by providing a unique type of protein glue that boosts multiple protein interactions along the ATR pathway PMID: 24990965
3. Data on crystal structure of BRCA1 binding with phosphopeptides suggest that C-terminal domain of BRCA1 interacts with ATRIP and BAAT1 with preferences for specific side chains; in ATRIP, phospho-Ser239 and Phe242 are the main interacting residues. PMID: 24073851
4. Data indicate that ATRIP is a direct target gene of HIF-1, and the increased ATRIP then activates the ATR signaling pathway under hypoxia. PMID: 23454212
5. our analysis exposes an overlapping clinical manifestation between the disorders but allows an expanded spectrum of clinical features for ATR-ATRIP Seckel Syndrome to be defined PMID: 23144622
6. as an ATR-associated kinase, Nek1 enhances the stability and activity of ATR-ATRIP before DNA damage, priming ATR-ATRIP for a robust DNA damage response PMID: 23345434
7. ATRIP may function outside the context of the canonical ATR damage signaling pathway during HSV-1 infection to participate in the viral life cycle. PMID: 20861269
8. data suggest that RPA-coated ssDNA is the critical structure at sites of DNA damage that recruits the ATR-ATRIP complex and facilitates its recognition of substrates for phosphorylation and the initiation of checkpoint signaling PMID: 12791985
9. there are at least two in vitro ATR-ATRIP DNA binding complexes, one which binds DNA with high affinity in an RPA-dependent manner and a second, which binds DNA with lower affinity in an RPA-independent manner PMID: 14724280
10. Collectively, our results suggest that the ATR-mediated phosphorylation of ATRIP at Ser-68 and -72 is dispensable for the initial response to DNA damage. PMID: 15451423
11. N-terminal domain of the ATRIP protein contributes to the cell cycle checkpoint by regulating the intranuclear localization of ATR PMID: 15527801
12. ATRIP is required for ATR accumulation at intranuclear foci induced by DNA damage. PMID: 15743907
13. ATRIP oligomerization is essential for the function of the ATM and Rad3-related (ATR)-ATRIP complex, which exists in higher order oligomeric states within cells PMID: 16027118
14. The expression of dimerization-defective ATRIP diminishes the maintenance of replication forks during treatment with DNA replication inhibitors. PMID: 16176973
15. support the idea of a multistep model for ATR activation that requires separable localization and activation functions of ATRIP PMID: 17339343
16. a direct physical interaction between BRCA1 and ATRIP is required for the checkpoint function of ATR PMID: 17616665
17. ATRIP is a CDK2 substrate, and CDK2-dependent phosphorylation of S224 regulates the ability of ATR-ATRIP to promote cell cycle arrest in response to DNA damage PMID: 17638878
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亞細胞定位：

Nucleus. Note=Redistributes to discrete nuclear foci upon DNA damage.
蛋白家族：

ATRIP family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 33499

OMIM: 606605

KEGG: hsa:84126

STRING: 9606.ENSP00000323099

UniGene: Hs.694840

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