Phospho-APLF (S116) Antibody

說明書

中文名稱：

磷酸化-APLF (S116)兔多克隆抗體
貨號：

CSB-PA008028
規格：

￥1090
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q8IW19
基因名：

APLF
別名：

2010301N04Rik antibody; AI452191 antibody; Aplf antibody; APLF_HUMAN antibody; Aprataxin and PNK-like factor antibody; Apurinic-apyrimidinic endonuclease APLF antibody; C2orf13 antibody; PNK and APTX like FHA protein antibody; PNK and APTX-like FHA domain-containing protein antibody; RGD1565557 antibody; XIP1 antibody; XRCC1 interacting protein 1 antibody; XRCC1-interacting protein 1 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from Human APLF around the phosphorylation site of S116.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
IHC	1:100-1:300
ELISA	1:5000

Protocols：

Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).
基因功能參考文獻：
1. characterization of the interaction of the APLF FHA domain with phosphorylated XRCC1 peptides PMID: 29059378
2. These data suggest functional requirements for Ku and XRCC4 in APLF-dependent NHEJ and a unique role for Ku as a factor required to facilitate the nuclear retention of APLF. PMID: 23689425
3. APLF promotes the assembly and activity of multi-protein Ku-DNA complexes containing all of the Non-homologous end joining (NHEJ) factors required for DNA ligation. PMID: 23178593
4. Polynucleotide kinase and aprataxin-like forkhead-associated protein (PALF) acts as both a single-stranded DNA endonuclease and a single-stranded DNA 3' exonuclease and can participate in DNA end joining in a biochemical system. PMID: 21885877
5. only a positive association (OR=1.58, 95% CI 1.05-2.46, P=0.027) was obtained for XRCC1 (Arg280His PMID: 21414327
6. APLF has a role in chromosomal DNA double-strand break repair. PMID: 21211721
7. The poly(ADP-ribose)-regulated protein APLF as a DNA-damage-specific histone chaperone. PMID: 21211722
8. The authors present solution structures of the two poly(ADP-ribose)-binding zinc finger modules of aprataxin and PNK-like factor (APLF), revealing a novel type of zinc finger. PMID: 20098424
9. These data identify APLF as a novel component of the cellular response to DNA strand breaks in human cells. PMID: 17353262
10. PALF is novel human AP endonuclease with conserved zinc-finger-like motifs involved in DNA strand break responses. PMID: 17396150
11. APLF is an ATM target that is involved in nonhomologous end-joining and facilitates double-strand break repair, likely via interactions with Ku and XRCC4-DNA ligase IV. PMID: 18077224
12. Study concludes that APLF can accumulate at sites of chromosomal damage via zinc finger-mediated binding to poly(ADP-ribose) and is a novel component of poly(ADP-ribose) signaling in mammalian cells. PMID: 18474613
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亞細胞定位：

Nucleus. Chromosome. Cytoplasm, cytosol.
蛋白家族：

APLF family
數據庫鏈接：

HGNC: 28724

OMIM: 611035

KEGG: hsa:200558

STRING: 9606.ENSP00000307004

UniGene: Hs.720369

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