PSMD14 Antibody

說明書

中文名稱：

PSMD14兔多克隆抗體
貨號：

CSB-PA644570
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA644570(PSMD14 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA644570(PSMD14 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 10%SDS-PAGE, Lysate: 50 μg, Lane 1-4: 293T cells, SKOV3 cells, NIH/3T3 cells, 231 cells, Primary antibody: CSB-PA644570(PSMD14 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

O00487
基因名：

PSMD14
別名：

26S proteasome non-ATPase regulatory subunit 14 antibody; 26S proteasome regulatory subunit rpn11 antibody; 26S proteasome-associated PAD1 homolog 1 antibody; 26S proteasome-associated PAD1 homolog antibody; PAD1 antibody; PAD1, yeast, homolog of antibody; POH1 antibody; Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody; Proteasome 26S subunit non ATPase 14 antibody; PSDE_HUMAN antibody; Psmd14 antibody; RPN11 antibody; Testis tissue sperm binding protein Li 69n antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Fusion protein of Human PSMD14
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.
基因功能參考文獻：
1. overexpression of RPN11 in breast cancer tissues was associated with an advanced clinical stage. Patients with tumors with high expression of RPN11 had worse prognosis. PMID: 28535005
2. Deubiquitylase POH1 stabilizes E2F1 protein through binding to and deubiquitylating E2F1 in liver cancer. PMID: 26510456
3. The data demonstrated that proteasomal POH1 is a key de-ubiquitinating enzyme that regulates ubiquitin conjugates generated in response to damage and that several aspects of the DNA double-strand break response are regulated by the proteasome. PMID: 22909820
4. Disorder of POH1 expression is involved in the onset of idiopathic nephrotic syndrome (INS), and confers multidrug resistance in children with INS PMID: 19419512
5. Down-regulation of PSMD14 results in decreased cell proliferation, cell cycle arrest and senescence. A comparative study with PSMB5, revealed that PSMB5 and PSMD14 have different effects on cell cycle, senescence and associated molecular events. PMID: 19732767
6. Ectopic expression of POH1 in HEK293 cells decreased the level of c-Jun ubiquitination, leading to significant accumulation of the protein and a corresponding increase in AP1-mediated gene expression PMID: 16569633
7. An intact zinc metalloproteinase motif of Poh1 is essential for cell viability and 26S proteasome function. PMID: 17237285
8. Specificity for K63-linked polyubiquitin is a common property of the JAMM/MPN+ family of deubiquitinating enzymes. PMID: 19214193
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蛋白家族：

Peptidase M67A family, PSMD14 subfamily
組織特異性：

Widely expressed. Highest levels in heart and skeletal muscle.
數據庫鏈接：

HGNC: 16889

OMIM: 607173

KEGG: hsa:10213

STRING: 9606.ENSP00000386541

UniGene: Hs.740477

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