PRKD3 Antibody

說明書

中文名稱：

PRKD3兔多克隆抗體
貨號：

CSB-PA145264
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA145264(PRKD3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA145264(PRKD3 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Human testis tissue, K562 cells, A549 cells, Raji cells, NIH/3T3 cells, Hela cells, Primary antibody: CSB-PA145264(PRKD3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

O94806
基因名：

PRKD3
別名：

EPK 2 antibody; EPK2 antibody; KPCD3_HUMAN antibody; nPKC nu antibody; nPKC-nu antibody; nPKCnu antibody; nu antibody; PKCnu antibody; PKD 3 antibody; PKD3 antibody; PRK D3 antibody; PRKCN antibody; PRKD 3 antibody; Prkd3 antibody; Protein kinase C antibody; Protein kinase C nu antibody; Protein kinase C nu type antibody; Protein kinase D3 antibody; Protein kinase EPK 2 antibody; Protein kinase EPK2 antibody; Serine threonine protein kinase antibody; Serine/threonine protein kinase D3 antibody; Serine/threonine-protein kinase D3 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthetic peptide of Human PRKD3
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
基因功能參考文獻：
1. Our findings suggest that abnormal PKD3 expression might contribute to hepatocellular carcinoma progression. Furthermore, high PKD3 expression predicts a poor prognosis in hepatocellular carcinoma patients after hepatectomy PMID: 28363424
2. The enriched pathway analysis reveals that PRKD3 regulates pathways contributing to multiple cancer related events, including cell cycle, migration and others. PMID: 28656000
3. None of the Polymorphous low-grade adenocarcinoma (PLGA) lacking PRKD1 somatic mutations or PRKD gene family rearrangements harboured somatic mutations in the kinase domains of the PRKD2 or PRKD3 genes. PMID: 26426580
4. This study discovered and characterized a novel, highly conserved N-terminal domain, comprising 92 amino acids, which mediates dimerization of Protein Kinase D (PKD) isoforms, PKD1, PKD2, and PKD3 monomers. PMID: 27662904
5. Studies indicate that the loss of protein kinase D PKD1 is thought to promote invasion and metastasis, while PKD2 and upregulated PKD3 to be positive regulators of proliferation. PMID: 26253275
6. Loss of PKD2 enhanced KC proliferative potential while loss of PKD3 resulted in a progressive proliferation defect, loss of clonogenicity and diminished tissue regenerative ability. PMID: 25802335
7. for the first time, PKD3 is implicated in the transcription activation of latent HIV-1 provirus, and our results revealed a molecular mechanism of prostratin-induced HIV-1 transcription via PKCepsilon/PKD3/NF-kappaB signaling pathway. PMID: 25136641
8. Activation of PKD2 and further increase of PKD3 activity leads to additional phosphorylation and inhibition of endogenous phosphatase slingshot 1L. PMID: 24840177
9. PKD3 in TNBC cells provides a molecular connection between the Golgi and endolysosomal compartments to enhance proliferative mTORC1-S6K1 signaling. PMID: 24337579
10. PKD3 negatively regulates human airway epithelial barrier formation and integrity through down-regulation of claudin-1, which is a key component of tight junctions. PMID: 24265314
11. PKD3 may contribute to the malignant progression of prostate cancer cells through negative regulation of MMP-7 expression. PMID: 20813660
12. PKD3 contributes to the proliferation and malignant growth of androgen-dependent prostate cancer cells in part by upregulating PSA expression. PMID: 20813663
13. We have identified PKD2 and PKD3, especially PKD3, as novel cell growth regulators in HCC1806 triple-negative breast cancer cells PMID: 23393329
14. PKD antagonists inhibited differentiation and viability of human hematopoietic stem cells, and had less HDAC phosphorylation, confirming the need for PKD-HDAC signaling in erythropoiesis. PMID: 22983445
15. phosphorylation of GIT1 on serine 46 by PKD3 represents a molecular switch by which GIT1 localization, paxillin trafficking, and cellular protrusive activity are regulated. PMID: 22893698
16. Data demonstrate the functional role of protein kinase D1 and protein kinase D3 in modulating physiological responses to Ox1R activation. PMID: 20621130
17. Novel isoforms regulate human keratinocyte differentiation by activating a p38 delta mitogen-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha. PMID: 12080077
18. PKCnu is an important component of signaling pathways downstream from novel PKC enzymes after B-cell receptor engagement PMID: 12506120
19. the effect of G protein-coupled receptor agonists on the regulatory properties, continuous shuttling, and intracellular distribution of Protein kinase C nu/protein kinase D3 PMID: 12676944
20. specificity of different PKD isoforms in regulating protein trafficking from the trans-Golgi network PMID: 14743217
21. PKD3 directly contributes to insulin-independent basal glucose uptake in L6 skeletal muscle cells. PMID: 15496505
22. Expression of constitutively activated PKCeta caused Golgi fragmentation. PMID: 15824133
23. the catalytic activity of PKD3 may regulate its nuclear import through autophosphorylation and/or interaction with another protein PMID: 16380377
24. The vesicular distribution may be attributed in part to the direct interaction between PKD3 and vesicle-associated membrane protein VAMP2, through which PKD3 may regulate VAMP2 vesicle trafficking by facilitating its recruitment to the target membrane. PMID: 17196367
25. A pathway comprising PKCs>Raf-1>MEK-1>ERK-1/-2 mediates the effect of gastrin on the CgA promoter, and strongly suggests that enhanced phosphorylation of Sp1 and CREB is crucial for CgA transactivation through the G protein-coupled CCK-B/gastrin receptor. PMID: 17889508
26. The data suggest that protein kinase C contributes to the phosphorylation of influenza PB1 and NS1 proteins which appears to be functionally relevant for both viral RNA polymerase activity and efficient viral replication. PMID: 19264651
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亞細胞定位：

Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, PKD subfamily
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 9408

OMIM: 607077

KEGG: hsa:23683

STRING: 9606.ENSP00000234179

UniGene: Hs.660757

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