PPP1R8 Antibody

說明書

中文名稱：

PPP1R8兔多克隆抗體
貨號：

CSB-PA618641DSR1HU
規格：

￥440
圖片：
Western blot
All lanes: PPP1R8 antibody at 4.73µg/ml
Lane 1: PC-3 whole cell lysate
Lane 2: HL60 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 39, 23, 14 kDa
Observed band size: 39 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA618641DSR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA618641DSR1HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA618641DSR1HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PPP1R8 Polyclonal antibody
Uniprot No.：

Q12972
基因名：

PPP1R8
別名：

Activator of RNA decay antibody; ARD 1 antibody; ARD-1 antibody; ARD1 antibody; Homolog of E.coli RNase E antibody; NIPP 1 antibody; NIPP-1 antibody; NIPP1 antibody; Nuclear inhibitor of protein phosphatase 1 antibody; nuclear inhibitor of protein phosphatase-1 alpha antibody; Nuclear subunit of PP1 antibody; PP1R8_HUMAN antibody; PPP1R8 antibody; PRO2047 antibody; Protein phosphatase 1 regulatory inhibitor subunit 8 antibody; Protein phosphatase 1 regulatory subunit 8 antibody; RNase E antibody; RNase E, E. coli, homolog of antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Nuclear inhibitor of protein phosphatase 1 protein (1-209AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.; Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
基因功能參考文獻：
1. NIPP1 could be activated by hypoxia and contributed to hypoxia-induced invasive and metastatic potential in hepatocellular carcinoma. PMID: 27644248
2. Data suggest overexpression of NIPP1 in HeLa cells causes induction of genes of mesenchymal lineage with reduction in cell proliferation; thus, key dephosphorylation events may be involved in epithelial-mesenchymal transition or transdifferentiation. PMID: 25907536
3. The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly PMID: 11909864
4. NIPP1 has a role in the nuclear targeting and/or retention of PP1 PMID: 15501817
5. Required for the global trimethylation of histone3 at lysine27 and is implicated in gene silencing by enhancer of zeste homolog (EZH)2. PMID: 17724462
6. NIPP1 is present in a complex with EED and EZH2 in vivo and has distinct binding sites for these proteins. PMID: 17804093
7. NMR structure of the NIPP1 FHA domain. PMID: 18253837
8. NIPP1 works as a molecular sensor for PP1 to recognize phosphorylated Sap155. PMID: 18842582
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亞細胞定位：

Nucleus. Nucleus speckle. Note=Primarily, but not exclusively, nuclear.; [Isoform Gamma]: Cytoplasm. Note=Found mainly in the cytoplasm.
組織特異性：

Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform
數據庫鏈接：

HGNC: 9296

OMIM: 602636

KEGG: hsa:5511

STRING: 9606.ENSP00000311677

UniGene: Hs.533474

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