POLDIP3 Antibody

中文名稱：

POLDIP3兔多克隆抗體
貨號：

CSB-PA871610LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate
All lanes: POLDIP3 antibody at 3.2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 43 kDa
Observed band size: 43 kDa

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871610LA01HU at dilution of 1:100

Immunofluorescence staining of Hela cells with CSB-PA871610LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating POLDIP3 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871610LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871610LA01HU (8μg) + Jurkat whole cell lysate (500μg)
Lane 3: Jurkat whole cell lysate (10μg)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) POLDIP3 Polyclonal antibody
Uniprot No.：

Q9BY77
基因名：

POLDIP3
別名：

46 kDa DNA polymerase delta interaction protein antibody; DNA polymerase delta interacting protein 3 antibody; KIAA1649 antibody; P46 antibody; PDIP3_HUMAN antibody; PDIP46 antibody; POLDIP3 antibody; Polymerase (DNA-directed), delta interacting protein 3 antibody; Polymerase delta interacting protein 46 antibody; Polymerase delta-interacting protein 3 antibody; RNA-binding protein P46 antibody; S6K1 Aly/REF-like target antibody; SKAR antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Polymerase delta-interacting protein 3 protein (100-221AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，POLDIP3 Antibody (CSB-PA871610LA01HU)，的標記方式是Non-conjugated。對于POLDIP3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA871610LB01HU	POLDIP3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA871610LC01HU	POLDIP3 Antibody, FITC conjugated
Biotin	CSB-PA871610LD01HU	POLDIP3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:20-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Is involved in regulation of translation. Is preferentially associated with CBC-bound spliced mRNA-protein complexes during the pioneer round of mRNA translation. Contributes to enhanced translational efficiency of spliced over nonspliced mRNAs. Recruits activated ribosomal protein S6 kinase beta-1 I/RPS6KB1 to newly synthesized mRNA. Involved in nuclear mRNA export; probably mediated by association with the TREX complex.
基因功能參考文獻：
1. Altered expression of PDIP46 or its mutation may affect Pol delta functions in vivo, and thereby be a nexus for altered genomic stability. PMID: 26819372
2. hnRNP-U inhibits TDP-43-mediated alterations in splicing of POLDIP3 mRNA. PMID: 25378556
3. PDIP3 and ZC11A associate with the human TREX complex in an ATP-dependent manner and function in mRNA export. PMID: 22928037
4. these findings establish critical and essential roles for SKAR in the regulation of mRNA translation of IFN-sensitive genes and induction of IFN-alpha biological responses. PMID: 25049393
5. Alteration of POLDIP3 splicing associated with loss of function of TDP-43 in tissues affected with amyotrophic lateral sclerosis (ALS) . PMID: 22900096
6. SKAR is a specific target of S6K1 and regulates cell growth. PMID: 15341740
7. This study showed that PDIP46 aka SKAR, a protein possessing one RNA recognition motif (RRM) and being a protein partner of both the p50 subunit of DNA polymerase delta and p70 ribosomal protein S6 kinase 1 (S6K1). PMID: 16984396
8. SKAR-mediated recruitment of activated S6K1 to newly processed mRNPs serves as a conduit between mTOR checkpoint signaling and the pioneer round of translation when cells exist in conditions supportive of protein synthesis. PMID: 18423201
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亞細胞定位：

Nucleus. Nucleus speckle. Cytoplasm. Note=Nucleocytoplasmic shuttling protein.
數據庫鏈接：

HGNC: 23782

OMIM: 611520

KEGG: hsa:84271

STRING: 9606.ENSP00000252115

UniGene: Hs.505802

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