PARD6B Antibody
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中文名稱：PARD6B兔多克隆抗體
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貨號(hào)：CSB-PA861174DSR1HU
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規(guī)格：￥440
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圖片：
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Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA861174DSR1HU at dilution of 1:100 -
IHC image of CSB-PA861174DSR1HU diluted at 1:325 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA861174DSR1HU at 1:108, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) PARD6B Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：PARD6B antibody; PAR6BPartitioning defective 6 homolog beta antibody; PAR-6 beta antibody; PAR-6B antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Partitioning defective 6 homolog beta protein (253-372AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, IF
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推薦稀釋比：
Application Recommended Dilution IHC 1:20-1:500 IF 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
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基因功能參考文獻(xiàn)：
- found that the polarity determinants Cdc42, Par6B, PKCzeta/iota and the structural proteins ezrin and phospho-ezrin were lost from the apical membrane and accumulated either in the cytoplasm or on the basal side of enterocytes in patients PMID: 26526116
- PAK4 phosphorylates Par6B at Ser143 blocking its interaction with Cdc42. PMID: 25662318
- Data conclude that Par6B and aPKC control mitotic spindle orientation in polarized epithelia and, furthermore, that aPKC coordinately regulates multiple processes to promote morphogenesis. PMID: 21300793
- This study demonstrates that controlled regulation of PAK4 is required for apical junction formation in lung epithelial cells and highlights potential cross-talk between two Cdc42 targets, PAK4 and Par6B. PMID: 20631255
- The ability of SRC family coactivators to regulate the expression of one of these genes, PARD6B/Par6, was confirmed by using cells individually depleted of SRC-1. PMID: 15677324
- Interaction between PAR-3 and the aPKC-PAR-6 complex is indispensable for apical domain development of epithelial cells. PMID: 19401335
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亞細(xì)胞定位：Cytoplasm. Cell membrane. Cell junction, tight junction.
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蛋白家族：PAR6 family
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組織特異性：Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.
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數(shù)據(jù)庫鏈接：
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