PAH1 Antibody

^{Rare Species}

說明書

中文名稱：

PAH1 Antibody
貨號(hào)：

CSB-PA333712XA01SVG
規(guī)格：

￥880
圖片：
Western Blot
Positive WB detected in: recombinant protein
All lanes:PAH1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 30.4 kDa
Observed band size: 30 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAH1 Polyclonal antibody
Uniprot No.：

P32567
基因名：

PAH1
別名：

Phosphatidic acid phosphohydrolase 1 (PAP1) (EC 3.1.3.4) (Protein SMP2) PAH1 PAP1 SMP2 YMR165C YM8520.14C
宿主：

Rabbit
反應(yīng)種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
免疫原：

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PAH1 protein (350-583aa)
免疫原種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
標(biāo)記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. Required for de novo lipid synthesis and formation of lipid droplets. Controles transcription of phospholipid biosynthetic genes and nuclear structure by regulating the amount of membrane present at the nuclear envelope. Involved in plasmid maintenance, in respiration and in cell proliferation.
基因功能參考文獻(xiàn)：
1. Site-specific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-637 plays a crucial role in Pah1 function. This work also demonstrated that the catalytic activity of Pah1 is required but is not sufficient for its in vivo functions PMID: 29066621
2. These results suggest that Pah1p plays an important role in vacuolar activity by negatively regulating the expression of V-ATPase genes. As such, we provide evidence to show the role of Pah1p in vacuolar acidification and fragmentation. PMID: 28756231
3. The expression of Pah1 with combined mutations of S705D and 7A, which mimic its phosphorylation by CKII and lack of phosphorylation by Pho85-Pho80, caused an increase in triacylglycerol content and lipid droplet number in cells expressing the Nem1-Spo7 phosphatase complex. PMID: 27044741
4. shortened chronological life span of the pah1Delta mutant along with its growth defect on non-fermentable carbon sources and hypersensitivity to hydrogen peroxide was suppressed by the loss of Dgk1 diacylglycerol kinase PMID: 26338708
5. proteolysis of Pah1 by the 20S proteasome is controlled by its phosphorylation state PMID: 25809482
6. Report functional conservation of PAP1 between yeast and human. PMID: 25475986
7. essential role of PAH1 in triacylglycerol synthesis throughout growth PMID: 24196957
8. Phosphorylation/dephosphorylation is a major mechanism by which Pah1p PAP activity is regulated. (Review) PMID: 22910056
9. Pah1p and PA phosphatase activity are critical for vacuole homeostasis and fusion. PMID: 22121197
10. the expression of PAH1-encoded phosphatidate phosphatase was induced by zinc deficiency. PMID: 22128164
11. Phosphatidate phosphatase activity plays key role in protection against fatty acid-induced toxicity in yeast PMID: 21708942
12. Data suggest that diacylglycerol generated by Pah1p is important for lipid droplet biogenesis. PMID: 21422231
13. Dephosphorylation of Pah1p by the Nem1p-Spo7p complex enables the amphipathic helix to anchor Pah1p onto the nuclear/ER membrane allowing the production of diacylglycerol for lipid biosynthesis. PMID: 20876142
14. Smp2 associates with the promoters of phospholipid biosynthetic enzymes in a Nem1-Spo7-dependent manner. Smp2 is a critical factor in coordinating phospholipid biosynthesis at the nuclear/ER membrane with nuclear growth during the cell cycle. PMID: 15889145
15. reports for the first time the identification of the S. cerevisiae PAH1 (previously known as SMP2) gene encoding a Mg2+-dependent PA phosphatase enzyme PMID: 16467296
16. PAP1 activity of the PAH1 gene product is essential for its roles in lipid metabolism and cell physiology. PMID: 17971454
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亞細(xì)胞定位：

Cytoplasm. Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
蛋白家族：

Lipin family
數(shù)據(jù)庫鏈接：

KEGG: sce:YMR165C

STRING: 4932.YMR165C

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