NUAK1 Antibody

說明書

中文名稱：

NUAK1兔多克隆抗體
貨號：

CSB-PA925362
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA925362(NUAK1 Antibody) at dilution 1/60, on the right is treated with synthetic peptide. (Original magnification: ×200)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

O60285
基因名：

NUAK1
別名：

NUAK1 antibody; ARK5 antibody; KIAA0537 antibody; OMPHK1 antibody; NUAK family SNF1-like kinase 1 antibody; EC 2.7.11.1 antibody; AMPK-related protein kinase 5 antibody; ARK5 antibody; Omphalocele kinase 1 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthetic peptide of Human NUAK1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
IHC	1:25-1:100

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
基因功能參考文獻：
1. NUAK1 is excessively expressed in NPC and may serve as a potential predictor of prognosis for NPC. PMID: 30121842
2. The elevated ARK5 expression was closely associated with cancer metastasis and patient survival, and it seemed to function in Gastric Cancer cells migration and invasion via epithelial-mesenchymal transition alteration, together with the alteration of the mTOR/p70S6k signals, Slug and SIP1. PMID: 28662499
3. ARK5 confers doxorubicin resistance in hepatocellular carcinoma by inducing epithelial-mesenchymal transition. PMID: 27126361
4. Nuak1 downregulation decreases tau levels in a human cell line. PMID: 27720485
5. High NUAK1 expression are correlated with epithelial-mesenchymal transition induction in head and neck cancer. PMID: 26882562
6. ARK5 was upregulated in ovarian cancer tissues, promoted epithelialmesenchymal transition and inhibited miR-1181 expression in ovarian cancer cells PMID: 26151663
7. MiR-145 functions as a tumor suppressor targeting NUAK1 in human intrahepatic cholangiocarcinoma. PMID: 26255969
8. Overexpression of NUAK1 is associated with disease-free survival and overall survival in patients with gastric cancer. PMID: 24943992
9. Data indicate that miR-96 suppresses the expression of (nua) kinase family 1 (NUAK1) by targeting its 3' untranslated region (3' UTR). PMID: 25242509
10. Results indicate that NUAK1 is excessively expressed in NSCLC and plays important roles in NSCLC invasion. PMID: 25412236
11. Expression of NUAK1 is controlled by cyclin-dependent kinase, PLK1, and the SCFbetaTrCP (Skp, Cullin and F-boxbetaTrCP) E3 ubiquitin ligase complex. PMID: 24785407
12. We demonstrate that miR-211 contributes to melanoma adhesion by directly targeting a gene, NUAK1. PMID: 23934065
13. Overexpression of ARK5 is associated with hepatocellular carcinoma. PMID: 23516026
14. High NUAK1 expression correlates with poor prognosis and involved in human nonsmall cell lung cancer cells migration and invasion. PMID: 23215946
15. ARK5 can promote glioma cell invasion by regulating cytoskeleton rearrangement and matrix metalloproteinase activation. PMID: 23063350
16. in human and murine cell lines, oncogenic levels of MYC establish a dependence on AMPK-related kinase 5 for maintaining metabolic homeostasis and for cell survival; ARK5 is an upstream regulator of AMPK and limits protein synthesis via inhibition of the mammalian target of rapamycin 1 (mTORC1) signalling pathway PMID: 22460906
17. ARK5 enhanced the invasive and metastatic potential of MDA-MB-231 cells under regulation by Akt. PMID: 22105900
18. NUAK1 and PPP1CC are identified as positional candidate loci for skeletal muscle strength phenotypes. PMID: 21750233
19. A novel role for NUAK1 in LKB1-related signaling pathways; NUAK1 can regulate cell proliferation and exert tumor suppression through direct interaction with p53. PMID: 21317932
20. the LKB1-NUAK pathway has roles in controlling myosin phosphatase complexes and cell adhesion PMID: 20354225
21. Cells that constitutively express NUAK1 suffer gross aneuploidies and show diminished expression of the genomic stability regulator LATS1, whereas depletion of NUAK1 with shRNA exerts opposite effects. PMID: 19927127
22. Data suggest that a novel AMPK family member, ARK5, is the tumor cell survival factor activated by Akt and acts as an ATM kinase under the conditions of nutrient starvation. PMID: 12409306
23. ARK5 suppresses the apoptosis induced by nutrient starvation and death receptors via inhibition of caspase 8 activation. PMID: 13679856
24. Results report that a novel AMPK catalytic subunit family member, ARK5, plays a key role in tumor malignancy downstream of Akt. PMID: 15060171
25. ARK5 negatively regulates procaspase-6 by phosphorylation at Ser257, leading to resistance to the FasL/Fas system. PMID: 15273717
26. overexpression of ARK5 is associated with tumor invasion and metastasis PMID: 15354411
27. ARK5 is a transcriptional target of the Large-MAF family through MARE sequence and that ARK5 may in part mediate the aggressive phenotype associated with c-MAF- and MAFB-expressing myelomas PMID: 16044163
28. NDR2 is an upstream kinase of ARK5 that plays an essential role in tumor progression through ARK5 PMID: 16488889
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亞細胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
組織特異性：

Expressed at high levels in heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung and liver. Highly up-regulated in colorectal cancer cell lines.
數據庫鏈接：

HGNC: 14311

OMIM: 608130

KEGG: hsa:9891

STRING: 9606.ENSP00000261402

UniGene: Hs.524692

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