NFS1 Antibody
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中文名稱：NFS1兔多克隆抗體
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貨號(hào)：CSB-PA896542ESR1HU
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規(guī)格：￥440
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圖片：
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Western blot
All lanes: NFS1 antibody at 6.21µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 51, 45 kDa
Observed band size: 51 kDa -
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA896542ESR1HU at dilution of 1:100 -
Immunoprecipitating NFS1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA896542ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA896542ESR1HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) NFS1 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：Cysteine desulfurase antibody; Cysteine desulfurase; mitochondrial antibody; HUSSY 08 antibody; IscS antibody; mitochondrial antibody; NFS1 antibody; NFS1 nitrogen fixation 1 antibody; NFS1 nitrogen fixation 1 homolog (S. cerevisiae) antibody; NFS1_HUMAN antibody; NIFS antibody; nitrogen fixation 1 (S. cerevisiae; homolog) antibody; nitrogen fixation gene 1 antibody; nitrogen-fixing bacteria S; homolog of antibody; nitrogen-fixing bacteria S-like protein antibody; OTTHUMP00000030799 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Cysteine desulfurase, mitochondrial protein (208-457AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 IHC 1:20-1:200 IP 1:200-1:2000 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor.
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基因功能參考文獻(xiàn)：
- FXN binds the NFS1-ISD11-ACP-ISCU complex (SDAU), to activate the desulfurase activity and Fe-S cluster biosynthesis. In the absence of FXN, the NFS1-ISD11-ACP (SDA) complex was reportedly inhibited by binding of recombinant ISCU PMID: 30031876
- analysis of the NFS1-ISD11-ACP (SDA) complex forms the core of the iron-sulfur (Fe-S) assembly complex and associates with assembly proteins ISCU2, frataxin (FXN), and ferredoxin to synthesize Fe-S clusters PMID: 28634302
- NFS1 maintains the iron sulfur clusters in proteins that are essential for protecting them from oxidative damage; inactivating NFS1 or iron sulfur clusters can trigger ferroptosis, a non-apoptotic form of cell death, in cancer cells PMID: 29168506
- human NFS1 was almost fully able to complement the role of IscS in Moco biosynthesis when its specific interaction partner protein MOCS3 from humans was also present. PMID: 28766335
- The NFS1/ISD11 complex further interacts with scaffold protein ISCU and regulator protein frataxin, thereby forming a quaternary complex for Fe-S cluster formation. PMID: 28271877
- FDX1 and FDX2 both bind NFS1 and donate electrons for iron-sulfur cluster biosynthesis. PMID: 28001042
- Molecular dynamics flexible fitting of protein structures docked into the EM map of the model revealed a [FXN(42-210)]24.[NFS1]24.[ISD11]24.[ISCU]24 complex, consistent with the measured 1:1:1:1 stoichiometry of its four components. PMID: 27519411
- Our findings highlight that the ISD11 R68A/R68L mutation display reduced affinity to form a stable subcomplex with NFS1, and thereby fails to prevent NFS1 aggregation resulting in impairment of the Fe-S cluster biogenesis PMID: 26342079
- The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity. PMID: 24217246
- NFS1 binds preferentially to the D-state of ISCU while mtHSP70 binds preferentially to the D-state of ISCU and HSC20 binds preferentially to the S-state of ISCU. PMID: 23940031
- the interaction of NFS1 and MOCS3 in the cytosol of human cells, is reported. PMID: 23593335
- Nfs1, the cysteine desulfurase responsible for providing sulfur for cluster formation, is required for the increased Isu stability occurring after disruption of cluster formation on or transfer from Isu PMID: 22689995
- the cytosolic form of ISCS is a functional cysteine desulfurase that can collaborate with cytosolic ISCU to promote de novo iron-sulfur cluster formation PMID: 16527810
- Results show that human Nfs1 is required inside mitochondria for efficient maturation of cellular iron/sulfur proteins. PMID: 16847322
- Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis PMID: 18650437
- Icp55 protease and its substrate Nfs1 appear to be dual distributed between the nucleus and mitochondria PMID: 19720832
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亞細(xì)胞定位：[Isoform Mitochondrial]: Mitochondrion.; [Isoform Cytoplasmic]: Cytoplasm. Nucleus.
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蛋白家族：Class-V pyridoxal-phosphate-dependent aminotransferase family, NifS/IscS subfamily
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組織特異性：Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas.
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數(shù)據(jù)庫(kù)鏈接：
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