NEDD1 Antibody

說明書

中文名稱：

NEDD1兔多克隆抗體
貨號：

CSB-PA822820DSR1HU
規格：

￥440
圖片：
Western blot
All lanes: NEDD1 antibody at 3.25μg/ml
Lane 1: Mouse small intestine tissue
Lane 2: Jurkat whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72, 63, 73 kDa
Observed band size: 72 kDa

Immunohistochemistry of paraffin-embedded human glioma using CSB-PA822820DSR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA822820DSR1HU at dilution of 1:100

Immunoprecipitating NEDD1 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA822820DSR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA822820DSR1HU (4μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) NEDD1 Polyclonal antibody
Uniprot No.：

Q8NHV4
基因名：

NEDD1
別名：

FLJ35902 antibody; GCP WD antibody; NEDD-1 antibody; NEDD1 antibody; NEDD1_HUMAN antibody; Neural precursor cell expressed developmentally down-regulated protein 1 antibody; Neural precursor cell expressed, developmentally down regulated gene 1 antibody; Protein NEDD1 antibody; TUBGCP7 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Protein NEDD1 protein (411-660AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
基因功能參考文獻：
1. One important function of Aurora A in mitotic cells is to promote microtubule nucleation around the chromatin by phosphorylating NEDD1, and thereby to promote functional spindle assembly. PMID: 23273898
2. The mice treated with small interfering (si) RNA targeting NEDD1 by the atelocollagen-mediated delivery system showed a significantly prolonged survival in a xenograft model of human gastric cancer. PMID: 23106787
3. Data suggest that the NEDD1/gamma-tubulin interaction is finely tuned by multiple phosphorylation events in the S557-S574 region and is critical for spindle assembly. PMID: 22595525
4. a direct interaction with NEDD1 regulates gamma-tubulin recruitment to the centrosome PMID: 20224777
5. GCP-WD (NEDD1) broadly mediates targeting of the gamma-tubulin ring complex to sites of microtubule nucleation and to the mitotic spindle, which is essential for spindle formation. PMID: 16378099
6. The NEED1 protein was characterized and its function in centriole duplication and spindle assembly determined. PMID: 16461362
7. Data propose NEDD1 may be a promising target for controlling cell proliferation, in particular if targeted in combination with Plk1 inhibitors. PMID: 19243593
8. Data suggest that sequential phosphorylation of Nedd1 by Cdk1 and Plk1 plays a role in targeting gamma tubulin ring complex to the centrosome by promoting the interaction of Nedd1 with the gammaTuRC component gamma-tubulin, during mitosis. PMID: 19509060
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
數據庫鏈接：

HGNC: 7723

OMIM: 600372

KEGG: hsa:121441

STRING: 9606.ENSP00000451211

UniGene: Hs.270084

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