NCK2 Antibody

中文名稱：

NCK2兔多克隆抗體
貨號：

CSB-PA015531HA01HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: NCK2 antibody at 8µg/ml
Lane 1: Mouse brain tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA015531HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA015531HA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA015531HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) NCK2 Polyclonal antibody
Uniprot No.：

O43639
基因名：

NCK2
別名：

Cytoplasmic protein NCK2 antibody; GRB4 antibody; Growth factor receptor bound protein 4 antibody; NCK adaptor protein 2 antibody; Nck-2 antibody; NCK2 antibody; NCK2_HUMAN antibody; NCKbeta antibody; Non-catalytic region of tyrosine kinase adaptor protein 2 antibody; Noncatalytic region of tyrosine kinase beta antibody; SH2/SH3 adaptor protein NCK-beta antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Cytoplasmic protein NCK2 protein (1-380AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產(chǎn)品，NCK2 Antibody (CSB-PA015531HA01HU)，的標記方式是Non-conjugated。對于NCK2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產(chǎn)品名稱	應用
HRP	CSB-PA015531HB01HU	NCK2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA015531HC01HU	NCK2 Antibody, FITC conjugated
Biotin	CSB-PA015531HD01HU	NCK2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling.
基因功能參考文獻：
1. This study provides evidence of a possible role of NCK2 as biomarker of ovarian cancer progression. PMID: 29218693
2. There was no difference in the Nck2 mRNA expression between the SLE patients and the healthy subjects. PMID: 29911835
3. Nck1 and Nck2 Interact with WTIP. Nck1/2 integrates nephrin with the Hippo kinase cascade through association with the adaptor protein WTIP. PMID: 27033705
4. Data suggest that PINCH1 and Nck2 critically participate in the regulation of cellular radiosensitivity and EGFR function and downstream signaling in a cellular model of human squamous cell carcinoma. PMID: 26004008
5. Tir-Intimin interaction recruits the Nck adaptor to a Tir tyrosine phosphorylated residue where it activates neural Wiskott-Aldrich syndrome protein (N-WASP). PMID: 25482634
6. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics. PMID: 24287595
7. NCK2 is involved in the susceptibility to opiates addiction. PMID: 23533358
8. The hNck2 SH3 domain also exhibited pH dependent monomer-dimer transition. PMID: 23524290
9. Data show that both HK2 and NCK2 are expressed in the retinal ganglion cell layer. PMID: 23349798
10. Nck2 effectively influences human melanoma phenotype progression. PMID: 21992144
11. p21-Activated kinase 3 (PAK3) protein regulates synaptic transmission through its interaction with the Nck2/Grb4 protein adaptor. PMID: 21949127
12. Nck2 is an adaptor protein composed of 3N-SH3 domains followed by a unique Cterminal SH2 domain. It interacts with PINCH in integrin signal transduction, cell migratio and survival. Review. PMID: 21880263
13. biophysical analysis of Nck2 SH3 domain PMID: 19956763
14. Nck-2 interacts with focal adhesion kinase and modulates cell motility. Using a mutational strategy, the formation of the Nck-2-FAK complex is mediated by interactions involving multiple SH2 and SH3 domains of Nck-2. PMID: 11950595
15. Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. PMID: 12110186
16. analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID: 15764601
17. Molecular model of the first SRC homology 3 domain of Nck2. PMID: 16604428
18. Nck1 (Nckalpha) and Nck2 (Nckbeta and Grb4): binding specificities of both SH2 domains are essentially indistinguishable PMID: 16636066
19. determined the NMR structures and dynamic properties of the hNck2 SH3 domains and to define their ligand binding preferences with nine proline-rich peptides derived from Wire, CAP-1, CAP-2, Prk, Wrch1, Wrch2, and Nogo PMID: 16752908
20. Data show that Nck (isoforms 1 and 2) as a component of the CReP/PP1c holophosphatase complex contributes to maintain eIF2alpha in a hypophosphorylated state, and modulates translation and eIF2alpha signaling in response to ER stress. PMID: 16835242
21. Demonstrate connection between septins/SOCS7/NCK signaling and the DNA damage response. PMID: 17803907
22. the adaptors Nck and ShcA influenced adherence of S. Typhimurium to non-phagocytic cells. PMID: 17906149
23. Data show that Nck forms a complex with an atypical PxxDY motif of the CD3epsilon tail, which encompasses Tyr166 within the activation motif and a T-cell receptor endocytosis signal. PMID: 18555270
24. Results point to NCK2 as a disease candidate gene and further supports the GLC1B locus as an important genomic region that is associated with the genetic predisposition to glaucoma. PMID: 18723748
25. independent roles and mechanisms of action of Nckalpha and Nckbeta in dermal fibroblast migration, which is critical for wound healing. PMID: 19242519
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亞細胞定位：

Cytoplasm. Endoplasmic reticulum.
組織特異性：

Ubiquitous.
數(shù)據(jù)庫鏈接：

HGNC: 7665

OMIM: 604930

KEGG: hsa:8440

STRING: 9606.ENSP00000233154

UniGene: Hs.529244

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