MRGPRX2 Antibody

說(shuō)明書(shū)

中文名稱：

MRGPRX2兔多克隆抗體
貨號(hào)：

CSB-PA839361LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, COLO205 whole cell lysate, HepG2 whole cell lysate
All lanes: MRGPRX2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa

Immunofluorescent analysis of MCF-7 cells using CSB-PA839361LA01HU at dilution of 1:200 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Western Blot
Positive WB detected in: Mouse pancreatic tissue lysate, Mouse Lung tissue lysate
All lanes: MRGPRX2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 40, 55 kDa

Immunofluorescence staining of Hela cell with CSB-PA839361LA01HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of MCF-7 cell with CSB-PA839361LA01HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

To prepare this MRGPRX2 polyclonal antibody, rabbits were first immunized with the human MRGPRX2 antigen, which stimulates the production of specific antibodies against the MRGPRX2 antigen. The antibodies are then collected and purified using protein G affinity chromatography, resulting in a purity greater than 95%.

The purified MRGPRX2 polyclonal antibody is then tested in ELISA, WB, and IF. In ELISA, the MRGPRX2 polyclonal antibody can be used as the primary antibody to specifically recognize and bind to the MRGPRX2 antigen from human and mouse samples. This MRGPRX2 antibody can be used to detect and visualize the human and mouse MRGPRX2 proteins in WB and IF, respectively.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) MRGPRX2 Polyclonal antibody
Uniprot No.：

Q96LB1
基因名：

MRGPRX2
別名：

MRGPRX2; MRGX2; Mas-related G-protein coupled receptor member X2
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Mas-related G-protein coupled receptor member X2 protein (286-330AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，MRGPRX2 Antibody (CSB-PA839361LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于MRGPRX2 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA839361LB01HU	MRGPRX2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA839361LC01HU	MRGPRX2 Antibody, FITC conjugated
Biotin	CSB-PA839361LD01HU	MRGPRX2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.
基因功能參考文獻(xiàn)：
1. Low levels of MRGPRX2 were detected in non-asthma lung mast cells, but its expression was significantly up-regulated in asthma lung mast cells. PMID: 29295703
2. the present study demonstrates that FceRI and MRGPRX2 function can be inversely regulated by SCF in an acute scenario, suggesting that the signaling requirements differ profoundly between the routes. PMID: 28859248
3. The authors discuss the possibility that MRGPRX2 responds to various as-yet-unidentified endogenous ligands that have specific characteristics, and propose that MRGPRX2 plays an important role in regulating inflammatory responses to endogenous harmful stimuli, such as protein breakdown products released from damaged or dying cells. PMID: 29484687
4. these peptides are identified as fragments of albumin. The isolated fragments activate MRGPRX2 and degranulate MRGPRX2 expressing LAD 2 cells in dose-dependent manner. The isolated basic peptides generated from human albumin are able to degranulate mast cells via the MRGPRX2. PMID: 28844982
5. The results showed a kind of mechanism that sinomenine-induced anaphylactoid reactions were mediated by MRGPRX2 via activating PLC molecular signaling pathways to provoke mast cells Ca(2+) mobilization and degranulation. PMID: 28987593
6. data suggest that mast cells play important roles in both gingival homeostasis and periodontal disease by orchestrating an interaction between gingival epithelial cells and neutrophils via MRGPRX2-mediated degranulation PMID: 28694291
7. MRGX2 is related to nociception, adrenal gland secretion and mast cell degranulation. (Review) PMID: 26106044
8. contributes to larger and longer lasting wheal reactions to intradermally injected neuropeptides in patients with chronic urticaria PMID: 24954276
9. Ectopic expression of MrgX2 in rat basophilic leukemia RBL-2H3 cells and murine bone marrow mast cells renders these cells responsive to human beta-defensin for degranulation. PMID: 23698749
10. identified MrgX2 as a novel G protein-coupled receptor for the antibacterial peptide LL-37 and demonstrated that unlike most G protein-coupled receptors it is resistant to agonist-induced receptor phosphorylation, desensitization, and internalization. PMID: 22069323
11. cortistatin-14 shown to be a high affinity ligand for G protein coupled receptor MrgX2 which is expressed in dorsal root ganglion PMID: 12915402
12. MRGX2 amino acid substitutions may alter the interactions between MRGX2 protein and its ligand, thus, potentially led to adaptive changes of the pain-perception-related nervous system during human evolution. PMID: 15862286
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

G-protein coupled receptor 1 family, Mas subfamily
組織特異性：

Mainly expressed in mast cells. Has a limited expression profile, both peripheral and within the central nervous system, with highest levels in dorsal root ganglion. Detected in blood vessels, scattered lymphocytes, and gastrointestinal ganglia (at protei
數(shù)據(jù)庫(kù)鏈接：

HGNC: 17983

OMIM: 607228

KEGG: hsa:117194

STRING: 9606.ENSP00000333800

UniGene: Hs.350566

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