MRC1 Antibody

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說明書

中文名稱：

MRC1兔多克隆抗體
貨號：

CSB-PA338680XA01SVG
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: recombinant protein
All lanes: MRC1 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P25588
基因名：

MRC1
別名：

Mediator of replication checkpoint protein 1 (DNA replication checkpoint mediator MRC1) MRC1 YCL061C YCL61C/YCL60C
反應種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
免疫原：

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) MRC1 protein (711-851aa)
免疫原種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Affinity-chromatography
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Required for normal DNA replication. Phosphorylated in response to DNA replication stress. Phosphorylation allows it to mediate the activation of RAD53.
基因功能參考文獻：
1. An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses. PMID: 29371596
2. Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1. PMID: 27956499
3. Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts. PMID: 18321795
4. Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1. PMID: 23165348
5. Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress. PMID: 22298423
6. Data show that that ISC1 controls cell morphology in combination with checkpoint regulators Swe1, Cdk1(CDC28), MRC1, TOF1, CSM3, RAD9 and Rad53. PMID: 21840863
7. cells deleted of MRC1, a gene implicated in replication fork stabilization and in the replication checkpoint pathway, maintained wild-type firing times despite over twofold lengthening of S phase PMID: 20219942
8. Data show that the Tof1/Csm3/Mrc1 checkpoint complex interacts directly with the MCM helicase during both replication fork progression and when the replication fork is stalled. PMID: 15755447
9. Mrc1 functions constitutively to promote normal replication fork progression. PMID: 16137624
10. Tof1p-Csm3p function differently from MRC1 and their complex counteracts the Rrm3p helicase to control replication termination of Saccharomyces cerevisiae PMID: 16418273
11. Mrc1 plays a specific role in DNA replication, promoting the Srs2 recruitment to proliferating cell nuclear antigen (PCNA) independently of checkpoint signaling. PMID: 16724109
12. Mrc1 may negatively regulate Cdc45 and MCM helicase to render stalled forks capable of resuming replication. PMID: 16849602
13. Study reports that in the absence of Mrc1, a component of the replication forks, telomeres of cdc13 or yku70 mutants exhibited increased degradation, while telomerase-negative cells displayed accelerated senescence. PMID: 17323081
14. analyzed sister-chromatid cohesion in double mutants of mrc1Delta, tof1Delta, and csm3Delta and identified additive cohesion defects that indicated the existence of at least two pathways that contribute to sister-chromatid cohesion PMID: 17483413
15. Mrc1 and Tof1 control the normal progression of DNA replication forks in distinct ways. PMID: 17652453
16. In unperturbed cells, Mrc1 interacts independently with both the N-terminal and C-terminal halves of Pol2 (Pol2N and Pol2C). PMID: 18851837
17. Data show that the replication checkpoint suppresses the formation of Rad52 foci in an Mrc1-dependent manner and prevents homologous recombination (HR) at chromosome breaks induced by the HO endonuclease. PMID: 19322196
18. Rad53 is activated by Mec1 through adaptor protein Mrc1 PMID: 19457865
19. Mec1-dependent, Rad53-independent phosphorylation of Mrc1 is required to establish a positive feedback loop that stabilizes Mec1 and the replisome at stalled forks. PMID: 19515819
20. An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1. PMID: 19620285
21. Csm3, Tof1, and Mrc1 form a heterotrimeric mediator complex that associates with DNA replication forks PMID: 19819872
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亞細胞定位：

Nucleus. Note=Associated with chromatin during S phase.
數(shù)據(jù)庫鏈接：

KEGG: sce:YCL061C

STRING: 4932.YCL061C

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