MCM3 Antibody

說明書

中文名稱：

MCM3兔多克隆抗體
貨號：

CSB-PA110553
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA110553(MCM3 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: K562 cells, Jurkat cells, hela cells, 293T cells, Primary antibody: CSB-PA110553(MCM3 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P25205
基因名：

MCM3
別名：

Cervical cancer proto oncogene 5 antibody; DNA polymerase alpha holoenzyme associated P1 antibody; DNA polymerase alpha holoenzyme associated protein P1 antibody; DNA polymerase alpha holoenzyme-associated protein P1 antibody; DNA replication factor MCM3 antibody; DNA replication licensing factor mcm3 antibody; HCC 5 antibody; HCC5 antibody; hRlf beta subunit antibody; Human cervical cancer proto oncogene 5 antibody; MCM 3 antibody; mcm3 antibody; MCM3 minichromosome maintenance deficient 3 antibody; MCM3_HUMAN antibody; MGC1157 antibody; Minichromosome maintenance complex component 3 antibody; Minichromosome maintenance deficient 3 antibody; Minichromosome maintenance protein 3 antibody; P1 h antibody; P1 MCM3 antibody; P1 Protein antibody; P1-MCM3 antibody; P1.h antibody; p102 antibody; P102 protein antibody; Replication licensing factor beta subunit antibody; RLF beta subunit antibody; RLF subunit beta antibody; RLFB antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Fusion protein of Human MCM3
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:5000
WB	1:500-1:2000
IHC	1:25-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
基因功能參考文獻：
1. this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma PMID: 27258565
2. High MCM3 expression is associated with bone metastasis and advanced human prostate cancer. PMID: 28424404
3. reveal the Minichromosome Maintenance Complex to be a critical and directly regulated node within the miR-183 signaling network in MYCN-amplified neuroblastoma cells. Randomly selected MCMs 3 and 5 were experimentally confirmed as direct targets of miR-183. PMID: 27239679
4. The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents. PMID: 28547962
5. These data establish new functions for KEAP1 within the nucleus and identify MCM3 as a novel substrate of the KEAP1-CUL3-RBX1 E3 ligase. PMID: 27621311
6. The aim of this study is to analyze the immunoexpression of Ki67, p53, MCM3 and PCNA markers in epithelial remnants of dental follicles of impacted teeth and to identify a possible correlation between the immunoexpression of these markers in dentigerous cysts and keratocystic odontogenic tumors. PMID: 27516012
7. High MCM-3 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
8. normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1 PMID: 25809478
9. The relatively lower MCM3 protein expression in FVPTC. PMID: 23821456
10. Expression of KB cell MCM3 was not affected by doxorubicin. PMID: 24344040
11. present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type PMID: 24324072
12. High MCM3 expression is associated with mucoepidermoid carcinomas and adenoid cystic carcinomas in salivary gland tumors. PMID: 23886132
13. Relatively lower MCM3 protein expression in follicular variant of papillary thyroid carcinoma comparing to classical type could be due to a different tumorigenic pathway favored in this type of tissue. PMID: 23821456
14. These findings provide validation of the utility of MCM3 expression as an independent biomarker for prognostication of patients with primary melanoma PMID: 22805320
15. excess of MCM3 up-regulates the phosphorylation of CHK1 Ser-345 and CDK2 Thr-14. PMID: 21965652
16. MCM3 gene expression is decreased in follicular variant of papillary thyroid carcinoma, contrary to expectation. PMID: 21509594
17. Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. PMID: 20545442
18. MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of papillary thyroid carcinoma. PMID: 19818763
19. The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with this protein PMID: 12226073
20. Elevated MCM3 protein expression is associated with neoplasms PMID: 15623617
21. MCM3 is overexpressed in human astrocytic tumors and elicits a cancer-restricted humoral immune response in some patients with brain neoplasms PMID: 15671553
22. ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
23. analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs PMID: 17244605
24. These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex. PMID: 18524952
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

MCM family
數據庫鏈接：

HGNC: 6945

OMIM: 602693

KEGG: hsa:4172

STRING: 9606.ENSP00000229854

UniGene: Hs.179565

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