MCC Antibody

中文名稱：

MCC兔多克隆抗體
貨號：

CSB-PA013571LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA013571LA01HU diluted at 1:500 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA013571LA01HU diluted at 1:500 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA013571LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MCC Polyclonal antibody
Uniprot No.：

P23508
基因名：

MCC
別名：

Colorectal mutant cancer protein antibody; CRCM_HUMAN antibody; DKFZp762O1615 antibody; FLJ38893 antibody; FLJ46755 antibody; MCC 1 antibody; MCC antibody; MCC1 antibody; Mutated in colorectal cancers antibody; Protein MCC antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Colorectal mutant cancer protein (479-611AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，MCC Antibody (CSB-PA013571LA01HU)，的標記方式是Non-conjugated。對于MCC Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA013571LB01HU	MCC Antibody, HRP conjugated	ELISA
FITC	CSB-PA013571LC01HU	MCC Antibody, FITC conjugated
Biotin	CSB-PA013571LD01HU	MCC Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity.
基因功能參考文獻：
1. Study described a novel tumor suppressor function of MCC in the regulation of E-cadherin mediated cell-cell adhesion in colorectal cancer cells. PMID: 29035389
2. Data suggest that millisecond dynamic changes in PDZ1 domain conformation are responsible for higher affinity of scribble PDZ1 for phosphorylated ligands; oligopeptide fragments of RPS6KA2 and MCC were used as ligands in these nuclear magnetic resonance chemical shift experiments. (RPS6KA2 = ribosomal protein S6 kinase 2; MCC = mutated in colorectal cancer protein) PMID: 29144123
3. MCC might confer alterative genetic susceptibility to colorectal cancer in individuals with schizophrenia promising to shed more light on the relationship between schizophrenia and cancer progression. PMID: 27226254
4. Our results indicate that in sharp contrast to its tumor suppressive role in colorectal cancer, MCC functions as an oncogene in B cells PMID: 25200342
5. cytoplasmic MCC-DBC1 interaction sequesters DBC1 away from the nucleus, thereby removing a brake on DBC1 nuclear targets, such as SIRT1 PMID: 24824780
6. targeted by miR-494, which is overexpressed in hepatocellular carcinoma PMID: 23913442
7. MCC regulates lamellipodia formation by binding to Scrib and its downstream partner Myosin-IIB in a multiprotein complex. PMID: 22480440
8. we have shown that promoter methylation of the APC gene does not extend to the neighbouring MCC gene in lung cancer, but LOH is found at both loci. PMID: 22542170
9. Promoter methylated MCC is associated with inflammatory bowel disease in colorectal cancer. PMID: 22213290
10. observed a significant association of the rs11283943 SNP with increased breast cancer risk in an Indian population PMID: 21279955
11. MCC is a nuclear, beta-catenin-interacting protein that can act as a potential tumor suppressor in the serrated colorectal cancer pathway by inhibiting Wnt/beta-catenin signal transduction. PMID: 18591935
12. Results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, beta-catenin and NHERF1/2. PMID: 19555689
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亞細胞定位：

Cell membrane. Cell projection, lamellipodium. Nucleus. Cytoplasm. Note=Colocalizes with actin at the leading edge of polarized cells.
蛋白家族：

MCC family
組織特異性：

Expressed in a variety of tissues.
數據庫鏈接：

HGNC: 6935

OMIM: 159350

KEGG: hsa:4163

STRING: 9606.ENSP00000386227

UniGene: Hs.593171

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