MAP2K3 (Ab-189) Antibody

說明書

中文名稱：

MAP2K3 (Ab-189)兔多克隆抗體
貨號：

CSB-PA563519
規格：

￥2024
圖片：
Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Ab-189) Antibody(left) or the same antibody preincubated with blocking peptide(right).

Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Ab-189) Antibody.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) MAP2K3 Polyclonal antibody
Uniprot No.：

P46734
基因名：

MAP2K3
宿主：

Rabbit
反應種屬：

Human,Rat
免疫原：

Peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:200
IF	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.
基因功能參考文獻：
1. Advanced glycation end products significantly activated ASK1, MKK3, and MKK6, which led to activation of p38 MAPK, resulting in upregulated fibrotic response in human coronary smooth muscle cells. PMID: 30305582
2. The study identifies MKK3 as a negative regulator of mitochondrial function and inflammatory responses to cigarette smoke and suggests that MKK3 could be a therapeutic target. PMID: 27717867
3. High MKK3 expression is associated with lung cancer. PMID: 28628118
4. miR-21 targets MKK3 in vivo and in vitro, inhibiting the downstream factors IL-6 and TNF-alpha, in ischemia pretreatment protection from ischemia-reperfusion induced kidney injury. PMID: 26149640
5. MKK3 overexpression upregulated the cyclin-dependent kinase inhibitors, p16 INK4A and p15 INK4B in hepatocellular carcinoma cells was Bim1, was downregulated following MKK3 overexpression. PMID: 26573508
6. Our results suggest that asthma is associated with MKK3 over-expression in CD8+ cells; we have also demonstrated that MKK3 may be critical for airway neutrophilia PMID: 24480516
7. MicroRNA-21 promotes hepatocellular carcinoma HepG2 cell proliferation through repression of mitogen-activated protein kinase-kinase 3. PMID: 24112539
8. study detected higher MKK3 activation in isolated peripheral blood mononuclear cells from septic patients compared with nonseptic controls PMID: 24487387
9. study concludes MAP2K3 is a reproducible obesity locus that may affect body weight via complex mechanisms involving appetite regulation and hypothalamic inflammation PMID: 23825110
10. miR-20a acts in a feedback loop to repress the expression of MKK3 and to negatively regulate the p38 pathway-mediated VEGF-induced endothelial cell migration and angiogenesis PMID: 22696064
11. the balance between MKK6 and MKK3 mediates p38 MAPK associated resistance to cisplatin in NSCLC PMID: 22164285
12. LFA-1-induced stabilization of ARE-containing mRNAs in T cells is dependent on HuR, and occurs through the Vav-1, Rac1/2, MKK3 and p38MAPK signaling cascade PMID: 21206905
13. MAP2K3 is identified as a protein to promote senescence in human breast epithelial cells. PMID: 21137025
14. Data provide evidence that p38 Map kinase (MAPK) pathway is activated leading to increased upregulation of mixed lineage kinase 3, MKK3/6, MSK1, and Mapkapk2, upon treatment of BCR/ABL expressing cells with dasatinib. PMID: 19672773
15. role in activating Mirk protein kinase PMID: 11980910
16. interacts with phospholipase c-beta 2 PMID: 12054652
17. role in pathway that promotes urokinase plasminogen activator mRNA stability in invasive breast cancer cells PMID: 12377770
18. TAK1- and MKK3-mediated activation of p38 are facilitated by Smad7 PMID: 12589052
19. MKK3 is selectively activated by the new subfamily of Ste20-like kinases. PMID: 13679851
20. a specific requirement for p150(Glued)/dynein/functional microtubules in activation of MKK3/6 and p38 MAPKs in vivo. PMID: 15375157
21. MAP kinase kinase3- and 6-dependent activation of the alpha-isoform of p38 MAP kinase is required for the cytoskeletal changes induced by neutrophil adherence and influences subsequent neutrophil migration toward endothelial cell junctions PMID: 15516490
22. H-Ras-specific activation of Rac-MKK3/6-p38 pathway has a role in invasion and migration of breast epithelial cells PMID: 15677464
23. MKK3 and MKK6 make individual contributions to p38 activation in fibroblast-like synoviocytes after cytokine stimulation PMID: 15778394
24. p38 mediates EGF receptor activation after oxidant injury; Src activates MMK3, which, in turn, activates p38; and the EGF receptor signaling pathway plays a critical role in renal epithelial cell dedifferentiation PMID: 15797859
25. Bax is phosphorylated by stress-activated JNK and/or p38 kinase and phosphorylation of Bax leads to mitochondrial translocation prior to apoptosis PMID: 16709574
26. Mitogen-activated protein kinase (MAPK) kinase 3 (MKK3) is a key activator of p38 MAPK in glioma; MKK3 activation is strongly correlated with p38 activation in vitro and in vivo. PMID: 17406030
27. Cytokine activation of MAPK14 and apoptosis is opposed by ACTN4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3. PMID: 17438131
28. as a mediator of SF- and Src-stimulated NF-kappaB activity. Finally, the Src/Rac1/MKK3/6/p38 and Src/TAK1/NF-kappaB-inducing kinase pathways exhibited cross-talk at the level of MKK3. PMID: 19047046
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相關疾病：

Defects in MAP2K3 may be involved in colon cancer.
蛋白家族：

Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
組織特異性：

Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
數據庫鏈接：

HGNC: 6843

OMIM: 602315

KEGG: hsa:5606

STRING: 9606.ENSP00000345083

UniGene: Hs.514012

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