LIG3 Antibody

說明書

中文名稱：

LIG3兔多克隆抗體
貨號：

CSB-PA002166
規格：

￥1090
圖片：
Western Blot analysis of MCF7 cells using DNA Ligase III Polyclonal Antibody

Western Blot analysis of K562 Jurkat NIH-3T3 cells using DNA Ligase III Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P49916
基因名：

LIG3
別名：

D11Wsu78e antibody; DNA ligase antibody; DNA ligase 3 antibody; DNA ligase III antibody; DNLI3_HUMAN antibody; LIG 3 antibody; LIG2 antibody; LIG3 antibody; Ligase II; DNA; ATP dependent antibody; Ligase III DNA ATP dependent antibody; MGC78176 antibody; OTTMUSP00000000875 antibody; Polydeoxyribonucleotide synthase [ATP] 3 antibody; Polydeoxyribonucleotide synthase [ATP] antibody; Polydeoxyribonucleotide synthase antibody; RP23-316C11.6 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from the Internal region of Human DNA Ligase III.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:10000

Protocols：

Western Blotting (WB) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.
基因功能參考文獻：
1. The homozygotes of rs1052536 TT were associated with an increased risk for NTDs than CC, and variants of rs1052536 T were associated with an increased risk of Neural Tube Defects. The stratified analysis showed that TT genotype of rs1052536 increased the risk of anencephaly and the T allele significantly increased the risk of cranial Neural Tube Defects. PMID: 30022792
2. single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I PMID: 28751376
3. The g.29661G>A and g.29059C>T polymorphisms of LIG3 may play a role in the keratoconus and Fuchs endothelial corneal dystrophy pathogenesis and can be considered as markers in these diseases. PMID: 25817347
4. A computational approach to determine susceptibility to cancer by evaluating the deleterious effect of nsSNP in XRCC1 gene on binding interaction of XRCC1 protein with ligase III. PMID: 26449312
5. In the context of tyrosine kinase-activated leukemias, c-MYC contributes to aberrant DNA repair through downstream targets LIG3 and PARP1 up-regulation. PMID: 25828893
6. Domains constituting the LigIII catalytic core collaborate and are essential for formation of a DNA-bridging intermediate by adenylated LigIII that positions a pair of blunt-ended duplex DNAs for efficient and specific intermolecular ligation. PMID: 26130724
7. there is an absolute requirement for fully functional DNA ligase III (LIG3), but not ligase IV (LIG4), to facilitate the escape from a telomere-driven crisis. PMID: 25127141
8. LIGIII plays a role in additional EJing repair pathway only in the absence of Ku. PMID: 24837021
9. Results show that overexpression of DNA ligase III in mitochondria improves mitochondrial base excision repair and enhances cell survival after oxidative stress. PMID: 24674627
10. data confirm previous work showing that Lig3 is required to maintain mtDNA integrity and function, and highlight a new function of ATM in regulating DNA Lig3 stability and consequently mtDNA repair PMID: 24342190
11. Human Mre11/human Rad50/Nbs1 and DNA ligase IIIalpha/XRCC1 protein complexes act together in an alternative nonhomologous end joining pathway. PMID: 21816818
12. Using our cohort of 480 breast cancer patients, we provide replicated evidence that a polymorphism near the LIG3 gene is associated with acute skin toxicity following radiotherapy. PMID: 21620500
13. results establish a role for Lig3 in mitochondria, but distinguish it from its interacting protein Xrcc1 PMID: 21390132
14. The collective results support a "jackknife model" in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. PMID: 20518483
15. The interaction with Lig3alpha is promoted by serine 81 that is located within a putative S/TQ site in the N-terminus domain of TDP1. PMID: 20009512
16. calpain-mediated proteolysis of DNA ligase III plays an essential role in DNA damage-induced cell death in human cells PMID: 11994275
17. In response to DNA damage, DNA ligase IIIalpha translocate from centrosomes to chromosomes. PMID: 15653642
18. XRCC1 stimulates Pol beta strand displacement activity and releases inhibition of Pol beta by DNA-bound Lig III if ligation is prevented. PMID: 16442856
19. the interaction of DNA ligase III and DNA polymerase gamma is required for proper maintenance of the mammalian mitochondrial genome. PMID: 17054425
20. Results describe the activity requirements for DNA ligases III and IV in the pathways of non-homologous DNA end joining. PMID: 17492771
21. key role for XRCC1-DNA ligase IIIalpha in maintenance of genomic integrity by nucleotide excision repair in both dividing and nondividing cells PMID: 17643379
22. analysis of DNA-binding and nick recognition modules in human DNA ligase III PMID: 18238776
23. Data show that CHIP-mediated degradation and DNA damage-dependent stabilization regulate base excision repair proteins XRCC1, DNA polymerase beta, and DNA ligase III. PMID: 18313385
24. siRNA mediated down-regulation of DNA ligase III in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
25. altered DNA double-strand break repair in CML cells is caused by the increased activity of an alternative nonhomologous end-joining repair pathway, involving DNA ligase IIIalpha and WRN PMID: 18524993
26. PMID 10207110 showed that translation initiation at the first ATG in the cDNA can lead to a mitochondrially-targeted isoform of the protein. PMID: 10207110
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亞細胞定位：

[Isoform 1]: Mitochondrion.; [Isoform 2]: Mitochondrion.; [Isoform 3]: Nucleus.; [Isoform 4]: Nucleus.
蛋白家族：

ATP-dependent DNA ligase family
組織特異性：

Testis, thymus, prostate and heart.
數據庫鏈接：

HGNC: 6600

OMIM: 600940

KEGG: hsa:3980

STRING: 9606.ENSP00000367787

UniGene: Hs.100299

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