LARS Antibody

中文名稱：

LARS兔多克隆抗體
貨號：

CSB-PA873736LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, U251 whole cell lysate
All lanes: LARS antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135, 129, 56 kDa
Observed band size: 135 kDa

IHC image of CSB-PA873736LA01HU diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA873736LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) LARS Polyclonal antibody
Uniprot No.：

Q9P2J5
基因名：

LARS
別名：

cytoplasmic antibody; Cytoplasmic leucyl tRNA synthetase antibody; Cytosolic leucyl tRNA synthetase antibody; FLJ10595 antibody; FLJ21788 antibody; hr025Cl antibody; HSPC 192 antibody; HSPC192 antibody; KIAA1352 antibody; LARS 1 antibody; Lars antibody; LARS1 antibody; Leucine translase antibody; Leucine tRNA ligase antibody; Leucine--tRNA ligase antibody; Leucyl tRNA synthetase cytoplasmic antibody; Leucyl-tRNA synthetase antibody; LeuRS antibody; LEUS antibody; LRS antibody; PIG 44 antibody; PIG44 antibody; Proliferation inducing gene 44 antibody; RNTLS antibody; SYLC_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Leucine--tRNA ligase, cytoplasmic protein (614-892AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，LARS Antibody (CSB-PA873736LA01HU)，的標記方式是Non-conjugated。對于LARS Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA873736LB01HU	LARS Antibody, HRP conjugated	ELISA
FITC	CSB-PA873736LC01HU	LARS Antibody, FITC conjugated
Biotin	CSB-PA873736LD01HU	LARS Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs.
基因功能參考文獻：
1. Leucyl-tRNA synthetase (LRS) is a leucine sensor of the mTORC1 pathway. PMID: 28963468
2. The results showed a decrease in autophagy on addition of leucine, demonstrating crosstalk between leucine sensing, LRS translocation, RagD interaction, and mTORC1 activation. PMID: 28882589
3. the KMSKS catalytic loop affects the aminoacylation and editing capacities of leucyl-tRNA synthetase PMID: 25817995
4. Lack of a CP1 hairpin in LeuRS led to complete loss of aminoacylation, amino acid activation, and tRNA binding; however, the mutants retained post-transfer editing. PMID: 25051973
5. the carboxy-terminal domain of human mitochondrial (mt) leucyl-tRNA synthetase can be used to correct mt dysfunctions caused by mt-tRNA mutations. PMID: 24413190
6. Identification of a mutation in LARS as a novel cause of infantile hepatopathy PMID: 22607940
7. This work demonstrates that LRS is a key mediator for amino acid signaling to mTORC1. PMID: 22424946
8. hcLeuRS can charge RNALeu with non-cognate amino acids and exclude the incorrect products by multiple editing pathways. PMID: 20805241
9. leucyl-tRNA synthetase requires its C-terminal domain for its interaction with arginyl-tRNA synthetase in the multi-tRNA synthetase complex PMID: 16055448
10. We identified a novel G3283A transition in the mitochondrial DNA tRNA(Leu (UUR)) gene in a patient with ptosis, ophthalmoparesis and hyporeflexia. PMID: 17363246
11. Results show that K600 in human leucyl-tRNA synthetase affects amino acid specificity and tRNA aminoacylation. PMID: 17378584
12. findings suggest that LARS1 may play roles in migration and growth of lung cancer cells, which suggest its potential implication in lung tumorigenesis PMID: 18446061
13. Study of crystal structures of the editing domain from 2 eukaryotic cytosolic LeuRS; shows a conserved structural core containing the active site for hydrolysis, with distinct bacterial, archeal, or eukaryotic peripheral insertions. PMID: 19426743
14. the introduction of bulky residues into the amino acid binding pocket failed to block deacylation of tRNA, indicating that the architecture of the amino acid binding pocket is different compared to that of other characterized LeuRSs PMID: 19702327
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相關疾病：

Infantile liver failure syndrome 1 (ILFS1)
亞細胞定位：

Cytoplasm.
蛋白家族：

Class-I aminoacyl-tRNA synthetase family
數據庫鏈接：

HGNC: 6512

OMIM: 151350

KEGG: hsa:51520

STRING: 9606.ENSP00000377954

UniGene: Hs.432674

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