KCNT1 Antibody

中文名稱：

KCNT1兔多克隆抗體
貨號：

CSB-PA686387LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA686387LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA686387LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) KCNT1 Polyclonal antibody
Uniprot No.：

Q5JUK3
基因名：

KCNT1
別名：

bA100C15.2 antibody; EIEE14 antibody; ENFL5 antibody; KCa4.1 antibody; KCNT1 antibody; KCNT1_HUMAN antibody; Potassium channel subfamily T member 1 antibody; Potassium channel, sodium activated subfamily T, member 1 antibody; Potassium channel, subfamily T, member 1 antibody; Sequence like a calcium-activated K+ channel antibody; SLACK antibody; Slo2.2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Potassium channel subfamily T member 1 protein (1026-1230AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，KCNT1 Antibody (CSB-PA686387LA01HU)，的標記方式是Non-conjugated。對于KCNT1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA686387LB01HU	KCNT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA686387LC01HU	KCNT1 Antibody, FITC conjugated
Biotin	CSB-PA686387LD01HU	KCNT1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro).
基因功能參考文獻：
1. G288S missense mutation, associated with seizures and neurodevelopmental delay resulted in larger whole cell K+ currents compared with wild-type KCNT1 currents. PMID: 28747464
2. Case report describing 3 infants with malignant migrating partial seizures with KCNT1 mutations accompanied by massive systemic to pulmonary collateral arteries. PMID: 28987752
3. Stimulation of Slack K(+) channels alters mass at the plasma membrane by triggering dissociation of Phactr-1. PMID: 27545877
4. In the present study, we evaluated two other potential mechanisms for stabilization of Slo2 channels in a closed state: (1) dewetting and collapse of the inner pore (hydrophobic gating) and (2) constriction of the inner pore by tight criss-crossing of the cytoplasmic ends of the S6 alpha-helical segments. PMID: 27682982
5. two de novo, heterozygous KCNT1 mutations were identified in two unrelated malignant migrating partial seizures probands. Both mutations induced a marked leftward shift in homomeric channel activation gating. PMID: 26784557
6. Better understanding of the mechanisms underlying KCNT1-related disease will produce further improvements in treatment of the associated severe seizure disorders. PMID: 26740507
7. The sodium sensitivity of these epilepsy causing mutants probably determines the [Na(+)]i concentration at which these mutants exert their pathological effects. PMID: 26725113
8. We demonstrate that KCNT1 mutations are highly pleiotropic and are associated with phenotypes other than nocturnal frontal lobe epilepsy and malignant migrating focal seizures of infancy. PMID: 26122718
9. This study demonstrate that KCNT1 mutations are strongly associated with early-onset epileptic encephalopathy. PMID: 26140313
10. Five de novo mutations were identified in four genes (SCNN1A, KCNJ16, KCNB2, and KCNT1) in three Brugada syndrome patients (20%) PMID: 25339316
11. Nine different mutations of the KCNT1 (Slack) Na(+)-activated K(+) channel give rise to three distinct forms of epilepsy. PMID: 25482562
12. Slick channels, in contrast to the similar Slack channels, are the only high-conductance K+ channels strongly sensitive to small changes in cell volume. PMID: 25347289
13. Genetic studies reveal two novel genes for Ohtahara Syndrome: KCNT1 and PIGQ. PMID: 24463883
14. Novel variations in KCNT1 do not allow prediction of functional phenotypes that might explain, at least in part, the symptoms of malignant migrating partial seizures of infancy (MMPSI). PMID: 24315024
15. This gene-wide tagging study revealed no association between KCNT1 17 common variations and susceptibility of GGEs or AEDs (anti-epileptic drugs) efficacy of genetic generalized epilepsies in Chinese population. PMID: 24279416
16. This study demonistrated that KCNT1 mutations implicated in epilepsy cause a marked increase in function PMID: 24591078
17. this study performed analysis of KCNT1 in two unrelated patients with malignant migrating partial seizures in infancy.Because the G-to-A transition was located at CG dinucleotide sequences as previously reported for KCNT1 mutations, the recurrent occurrence of de novo KCNT1 mutations indicated the hot spots of these locations. PMID: 24029078
18. Mutations in KCNT1 cause a severe form of ADNFLE and sporadic NFLE. PMID: 23086396
19. Our data identify KCNT1 as a major disease-associated gene in Malignant migrating partial seizures of infancy . PMID: 23086397
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相關疾病：

Epileptic encephalopathy, early infantile, 14 (EIEE14); Epilepsy, nocturnal frontal lobe, 5 (ENFL5)
亞細胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

Potassium channel family, Calcium-activated (TC 1.A.1.3) subfamily, KCa4.1/KCNT1 sub-subfamily
組織特異性：

Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle.
數據庫鏈接：

HGNC: 18865

OMIM: 608167

KEGG: hsa:57582

STRING: 9606.ENSP00000360822

UniGene: Hs.104950

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