ITSN1 Antibody

中文名稱：

ITSN1兔多克隆抗體
貨號：

CSB-PA621885LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: 293T whole cell lysate
All lanes: ITSN1 antibody at 5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 196, 138, 130, 188, 116, 195, 189, 134, 127, 126, 105 kDa
Observed band size: 196 kDa

IHC image of CSB-PA621885LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ITSN1 Polyclonal antibody
Uniprot No.：

Q15811
基因名：

ITSN1
別名：

Human intersectin SH3 domain containing protein SH3P17 antibody; Intersectin 1 SH3 domain protein antibody; intersectin 1 short form variant 3 antibody; intersectin 1 short form variant, 11 antibody; Intersectin short form antibody; intersectin short variant 12 antibody; Intersectin-1 antibody; Intersectin1 antibody; ITSN 1 antibody; ITSN antibody; itsn1 antibody; ITSN1_HUMAN antibody; MGC134948 antibody; MGC134949 antibody; OTTHUMP00000067855 antibody; OTTHUMP00000067856 antibody; OTTHUMP00000067857 antibody; OTTHUMP00000194810 antibody; OTTHUMP00000194811 antibody; OTTHUMP00000194812 antibody; OTTHUMP00000206688 antibody; SH3 domain containing protein 1A antibody; SH3 domain protein 1A antibody; SH3 domain-containing protein 1A antibody; SH3D1A antibody; SH3P17 antibody; Src homology 3 domain containing protein antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Intersectin-1 protein (773-916AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ITSN1 Antibody (CSB-PA621885LA01HU)，的標記方式是Non-conjugated。對于ITSN1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA621885LB01HU	ITSN1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA621885LC01HU	ITSN1 Antibody, FITC conjugated
Biotin	CSB-PA621885LD01HU	ITSN1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery. Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex. Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits. Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1.; Plays a role in synaptic vesicle endocytosis in brain neurons.
基因功能參考文獻：
1. WIP enhances the interaction of N-WASP with ITSN1 and promotes ITSN1/beta-actin association. PMID: 29958948
2. Here, we discover that intersectin-s binds DENND2B, a guanine nucleotide exchange factor for the exocytic GTPase Rab13, and this interaction promotes recycling of ligand-free EGFR to the cell surface. Our study thus reveals a novel mechanism controlling the fate of internalized EGFR with important implications for cancer. PMID: 29030480
3. study reports that vaccinia virus A36 contains three NPF motifs that interact with the Eps15 homology (EH) domains in intersectin-1 and Eps15 to promote the release of virus from infected cells and their subsequent spread PMID: 27670116
4. Silencing ITSN1 in neuroblastoma cells led to decreased tumor growth in an orthotopic mouse model. Orthotopic animal models can provide insight into the role of ITSN1 pathways in neuroblastoma tumorigenesis. PMID: 28787396
5. These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion. PMID: 28161632
6. Abnormal expression of the intersectin1-L protein in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy. PMID: 25783631
7. Study suggests critical roles of ITSN1-S in malignant glioma proliferation, indicating a potential usage of ITSN1-S in the therapeutic intervention as a novel molecular target PMID: 25832561
8. Our observations suggest that ITSN1 is an important general regulator of Cdc42-, Nck- and N-WASP-dependent actin polymerisation PMID: 24284073
9. ITSN1 and ITSN2 bind similar proline-rich ligands but are differentially recognized by SH2 domain-containing proteins. PMID: 23936226
10. Silencing ITSN1 significantly inhibits the anchorage independent growth of tumor cells in vitro. PMID: 22266851
11. the neuron-specific isoform of the stable tubule-only polypeptide (STOP) interacts with SH3A domain of ITSN1. PMID: 22750298
12. ITSN-1s, via its SH3A domain has the unique ability to regulate dyn2 assembly-disassembly and function during endocytosis PMID: 21129155
13. ITSN1 has two isoforms: ITSN1-long and ITSN1-short. siRNA-mediated down regulation of ITSN1-short inhibits migration and invasion of glioma cells. PMID: 21503949
14. study reveals a link between overexpression of specific ITSN1 isoforms and behavioral phenotypes PMID: 21876463
15. This novel mammalian ITSN1 isoform possesses a significantly altered domain structure and performs specific protein-protein interactions. PMID: 21712076
16. Downregulation of intersectin-1s induces apoptosis of brain glioblastoma cells. PMID: 20493827
17. Studied the inhibitory mechanism of ITSN1L, & id'd a novel short amino acid motif which mediates autoinhibition. Found this motif is located in the SH3 DH linker region, and show that W1221 acts as key residue in establishing inhibitory interaction. PMID: 20842712
18. These findings expand the role of ITSN1 as a scaffolding molecule bringing together components of endocytic complexes. PMID: 20946875
19. The identification of fifteen novel transcriptional isoforms of the human ITSN1 gene with full-length coding sequences , is reported. PMID: 19777371
20. microexons provide a mechanism for the control of tissue-specific interactions of ITSN1 and Src with their partners. PMID: 20659428
21. intersectin interaction with dynamin was important in regulating the fission and internalization of caveolae PMID: 12960435
22. review of roles of ITSN1 and DSCR1 in Down syndrome, Alzheimer's disease, endocytosis and vesicle trafficking PMID: 16442855
23. Results extend the current role of intersectin-1L in endocytosis to a function in exocytosis and support the idea that intersectin-1L is an adaptor that coordinates exo-endocytotic membrane trafficking in secretory cells. PMID: 16874303
24. ITSN forms a complex with Cbl in vivo mediated by the Src homology (SH) 3 domains binding to the Pro-rich COOH terminus of Cbl. This interaction stimulates the ubiquitylation and degradation of the activated EGFR. PMID: 16914641
25. findings demonstrate a novel role of ITSN-1s as a negative regulator of the mitochondrial pathway-dependent apoptosis secondary to activation of the Erk1/2 survival signaling pathway PMID: 17405881
26. These results demonstrate that alternative splicing leads to the formation of two pools of ITSN1 with potentially different properties in neurons, affecting ITSN1 function as adaptor protein. PMID: 18539136
27. These data provide a molecular link between SHIP2 and ITSN1 which are involved in receptor endocytosis regulation. PMID: 18692052
28. Intersectin 1 forms a complex with adaptor protein (SH3KBP1 protein), implicated in downregulation of receptor tyrosine kinases. PMID: 19166927
29. The higher exchange activity of ITSN1L towards the GDP-bound conformation of Cdc42 could represent an evolutionary adaptation of this GEF that ensures nucleotide exchange towards the formation of the signalling-active GTP-bound form of Cdc42. PMID: 19356586
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亞細胞定位：

Endomembrane system. Cell junction, synapse, synaptosome. Cell projection, lamellipodium. Cell membrane. Membrane, clathrin-coated pit. Recycling endosome. Endosome. Cytoplasmic vesicle.; [Isoform 2]: Cytoplasm. Endomembrane system. Nucleus envelope.; [Isoform 5]: Endomembrane system.
組織特異性：

Isoform 1 is expressed almost exclusively in the brain. Isoform 2 is detected in brain, spleen, lung, liver, heart, skeletal muscle and kidney. Isoform 5 is primarily expressed in brain, spleen, lung and kidney (at protein level). Isoform 1 and isoform 2
數據庫鏈接：

HGNC: 6183

OMIM: 602442

KEGG: hsa:6453

STRING: 9606.ENSP00000370719

UniGene: Hs.160324

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