H1F0 (Ab-101) Antibody
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中文名稱：H1F0 (Ab-101)兔多克隆抗體
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貨號：CSB-PA010087OA101nme1HU
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規(guī)格：￥660
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圖片：
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Western Blot
Positive WB detected in: Rat spleen tissue
All lanes: H1F0 antibody at 0.85μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21, 20 kDa
Observed band size: 21 kDa -
IHC image of CSB-PA010087OA101nme1HU diluted at 1:50 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA010087OA101nme1HU at 1:1, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5μg anti-H1F0 (CSB-PA010087OA101nme1HU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) H1F0 Polyclonal antibody
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Uniprot No.：
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基因名：H1F0
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別名：H1 histone family member 0 antibody; H1(0) antibody; H10 antibody; H10_HUMAN antibody; h1f0 antibody; H1FV antibody; Histone H1'' antibody; Histone H1(0) antibody; Histone H1.0 antibody; Histone H10 antibody; Histone H5 antibody; MGC5241 antibody; N-terminally processed antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Rat
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免疫原：Peptide sequence around site of Lys (101) derived from Human Histone H1.0
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：Antigen Affinity Purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, ChIP
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推薦稀釋比：
Application Recommended Dilution WB 1:50-1:500 IHC 1:20-1:200 IF 1:1-1:10 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The histones H1.0 are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
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基因功能參考文獻：
- H1.X moves more rapidly than other linker histones in vivo Domain swapping between H1.0 and H1.X suggests that the globular domain (GD) and C-terminal domain (CTD) of H1.X independently contribute to the dynamic behavior of H1.X. PMID: 27528617
- this study shows that dynamic epigenetic states defined by the linker histone H1.0 determine which cells within a tumor can sustain the long-term cancer growth. PMID: 27708074
- The N-terminal domain contributes toward the differential chromatin binding affinity, whereas the C-terminal domain contributes toward distinct nucleosomal interface of isotypes H10 and H1c. PMID: 22334665
- nuclear transport of H1 histones requires a heterodimeric nuclear import receptor PMID: 12080050
- H1(0)histone may be an important factor in normal DC differentiation. Tumor-derived factors may inhibit DC differentiation by affecting H1(0) expression. PMID: 12149419
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亞細胞定位：Nucleus. Chromosome. Note=The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
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蛋白家族：Histone H1/H5 family
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數(shù)據(jù)庫鏈接：
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