GTF2B Antibody

中文名稱：

GTF2B兔多克隆抗體
貨號：

CSB-PA010001HA01HU
規格：

￥440
圖片：
Western blot
All lanes: GTF2B antibody at 2µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA010001HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010001HA01HU at dilution of 1:100

Immunofluorescent analysis of PC-3 cells using CSB-PA010001HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) GTF2B Polyclonal antibody
Uniprot No.：

Q00403
基因名：

GTF2B
別名：

General transcription factor IIB antibody; General transcription factor TFIIB antibody; gtf2b antibody; RNA polymerase II transcription factor IIB antibody; S300 II antibody; S300-II antibody; TF IIB antibody; TF2B antibody; TF2B_HUMAN antibody; TFIIB antibody; Transcription initiation factor IIB antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Transcription initiation factor IIB protein (1-316AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，GTF2B Antibody (CSB-PA010001HA01HU)，的標記方式是Non-conjugated。對于GTF2B Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA010001HB01HU	GTF2B Antibody, HRP conjugated	ELISA
FITC	CSB-PA010001HC01HU	GTF2B Antibody, FITC conjugated
Biotin	CSB-PA010001HD01HU	GTF2B Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Exhibits also autoacetyltransferase activity that contributes to the activated transcription.
基因功能參考文獻：
1. a hand-off model in which Ssu72 mediates a conformational transition in TFIIB, accounting for the role of Ssu72 in transcription reinitiation, gene looping, and promoter-terminator cross-talk. PMID: 29158257
2. binding of specific DNA sequences changes the protein structure and dynamics, and TFIIB may exist in two conformational states PMID: 26284261
3. The role of zinc at Cys3His1 binding site in the absence of TFIIB protein folding PMID: 26016528
4. these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects. PMID: 24441171
5. Association of the winged helix motif of the TFIIEalpha subunit of TFIIE with either the TFIIEbeta subunit or TFIIB distinguishes its functions in transcription. PMID: 25492609
6. TFIIB was involved in the proliferation and growth of HCC cells. PMID: 23055019
7. a mode of phospho-TFIIB-independent transcriptional regulation that prioritizes the transcription of p53-target genes during cellular stress. PMID: 23115335
8. Data show that TFIIF has an important role in stabilizing TFIIB within the PIC and after transcription initiates. PMID: 21896726
9. a six-amino acid human TFIIB tip region is needed for appropriate levels of serine 5 C-terminal domain phosphorylation and mRNA capping and for retention of the required elongation factor TFIIF. PMID: 20880846
10. phosphorylation of TFIIB at serine 65 is a critical event in transcription that links the gene promoter and terminator and triggers initiation by RNA pol II PMID: 20226668
11. interaction with vitamin D receptor helix H10 residues PMID: 12529369
12. Data show that human TATA box binding protein (TBP) can use a shared surface to interact with two different transcription factor IIB (TFIIB) family members to initiate transcription by different RNA polymerases. PMID: 12535529
13. The groove in the first cyclin repeat of TFIIB is identified as the FCP1-binding site of TFIIB core domain (TFIIBc), consisting of several hydrophobic and several basic amino acids. PMID: 12578358
14. lack of role in bending of TATA-binding protein simultaneously with promotoer DNA PMID: 12791683
15. TFIIB interacts with SP1/SP3 at the SP1 site, besides its association with EAR3 and the TATA-less core promoter region. PMID: 12972613
16. in addition to its role in regulating TBP binding to a TATA box, the TBP surface is unexpectedly involved in TBP association with all three TFIIB family members PMID: 14585974
17. The structural role of Zn2+ binding to the the zinc-ribbon domain of TFIIB was analysed. PMID: 14641108
18. Results identify a small nonconserved surface of the transcription factor TFIIB zinc ribbon that is required for RNA polymerase II (pol II) transcription in vivo and for different types of basal pol II transcription in vitro. PMID: 15024075
19. A TFIIB conformational change is critical for the formation of activator-dependent transcription complexes. PMID: 15037660
20. Collapse of the transcription bubble defines the RNA polymerase II promoter clearance transition. PMID: 15989968
21. high level of TFIIB can bypass the Mediator requirement for basal transcription and pol II recruitment in nuclear extract PMID: 16595664
22. Together, our results support a mode of preinitiation complex assembly in which TFIIB/RNA polymerase II recruitment to the promoter occurs in vivo. PMID: 16878124
23. TFIIB binding is required for the interferon antagonist effect exerted by Thogoto virus M protein. PMID: 18768974
24. slipping at juncture is induced by TATA-binding protein and transcription factor IIB and requires a TATA box but not a transcription factor IIB recognition sequence PMID: 19193635
25. TFIIF in HeLa cell nuclear extract helps to rescue the inactive mutations by interacting with either the B-finger or another component of the initiation complex that is influenced by the B-finger PMID: 19590095
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

TFIIB family
組織特異性：

Expressed in the inner cell mass forming the embryoblast. Not detected in cells from the outer thin layer trophoblast (at protein level).
數據庫鏈接：

HGNC: 4648

OMIM: 189963

KEGG: hsa:2959

STRING: 9606.ENSP00000359531

UniGene: Hs.481852

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