GPR12 Antibody
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中文名稱：GPR12兔多克隆抗體
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貨號：CSB-PA009742LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: K562 whole cell lysate
All lanes: GPR12 antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa -
IHC image of CSB-PA009742LA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells with CSB-PA009742LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) GPR12 Polyclonal antibody
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Uniprot No.：
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基因名：
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別名：GPR12; G-protein coupled receptor 12
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human G-protein coupled receptor 12 protein (1-48AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，GPR12 Antibody (CSB-PA009742LA01HU)，的標記方式是Non-conjugated。對于GPR12 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
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靶點詳情
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功能：Promotes neurite outgrowth and blocks myelin inhibition in neurons. Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production.
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基因功能參考文獻：
- Since we have demonstrated that CBD is an inverse agonist for GPR12, this provides novel mechanism of action for CBD, and an initial chemical scaffold upon which highly potent and efficacious agents acting on GPR12 may be developed with the ultimate goal of blocking cancer metastasis. PMID: 28888984
- GPR12 may play a role in cell proliferation and survival PMID: 22430950
- control of gene expression in vascular endothelial cells in the presence of fluid shear stress and classifies it as a sphingosine 1-phosphate receptor PMID: 12649592
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亞細胞定位：Cell membrane; Multi-pass membrane protein.
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蛋白家族：G-protein coupled receptor 1 family
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數據庫鏈接：
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