GNAT3 Antibody

中文名稱：

GNAT3兔多克隆抗體
貨號：

CSB-PA009600LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA009600LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA009600LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) GNAT3 Polyclonal antibody
Uniprot No.：

A8MTJ3
基因名：

GNAT3
別名：

GDCA antibody; Ggust antibody; Gnat 3 antibody; GNAT3 antibody; GNAT3_HUMAN antibody; Gtn antibody; Guanine nucleotide binding protein alpha transducing 3 antibody; Guanine nucleotide binding protein G(t) subunit alpha 3 antibody; Guanine nucleotide-binding protein G(t) subunit alpha-3 antibody; Gustducin alpha 3 antibody; Gustducin alpha 3 chain antibody; Gustducin alpha-3 chain antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Guanine nucleotide-binding protein G(t) subunit alpha-3 protein (2-354AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，GNAT3 Antibody (CSB-PA009600LA01HU)，的標記方式是Non-conjugated。對于GNAT3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA009600LB01HU	GNAT3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA009600LC01HU	GNAT3 Antibody, FITC conjugated
Biotin	CSB-PA009600LD01HU	GNAT3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.
基因功能參考文獻：
1. (GNAT3, 11 SNPs) gene, located within the 1-LOD support interval region for their association with MS and its related traits. Several SNPs were associated with MS and its related traits. Remarkably. PMID: 22456541
2. The sweet taste receptors (alpha-gustducin and T1R3) are involved in glucose-stimulated secretion of GLP-1 and PYY. PMID: 21324568
3. Observational study of gene-disease association. (HuGE Navigator) PMID: 20858777
4. Observational study of gene-disease association. (HuGE Navigator) PMID: 20660057
5. the alpha-subunit of the taste-specific G protein gustducin is expressed prominently in cells of the human colon PMID: 16728727
6. Axonemal-associated localization within the midpiece and principal piece of human spermatozoa raises the possibility that this G protein alpha-subunit may process intracellular signals controlling sperm motility. PMID: 17021831
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亞細胞定位：

Cytoplasm. Note=Dual distribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm.
蛋白家族：

G-alpha family, G(i/o/t/z) subfamily
組織特異性：

Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa.
數據庫鏈接：

HGNC: 22800

OMIM: 139395

KEGG: hsa:346562

STRING: 9606.ENSP00000381339

UniGene: Hs.335049

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