GLIS2 Antibody

中文名稱：

GLIS2兔多克隆抗體
貨號：

CSB-PA861185LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA861185LA01HU diluted at 1:350 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA861185LA01HU at 1:116, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) GLIS2 Polyclonal antibody
Uniprot No.：

Q9BZE0
基因名：

GLIS2
別名：

FLJ38247 antibody; GLI similar 2 antibody; GLI-similar 2 antibody; GLIS 2 antibody; GLIS family zinc finger 2 antibody; glis2 antibody; GLIS2_HUMAN antibody; Kruppel like zinc finger protein GLIS2 antibody; Neuronal Krueppel-like protein antibody; Zinc finger protein GLIS2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Zinc finger protein GLIS2 protein (332-505AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，GLIS2 Antibody (CSB-PA861185LA01HU)，的標記方式是Non-conjugated。對于GLIS2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA861185LB01HU	GLIS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861185LC01HU	GLIS2 Antibody, FITC conjugated
Biotin	CSB-PA861185LD01HU	GLIS2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation mediated by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.
基因功能參考文獻：
1. CBFA2T3-GLIS2 fusion is associated with acute megakaryoblastic leukemia in non-Down syndrome. PMID: 28063190
2. specific interference with ETO2-GLIS2 oligomerization reverses the transcriptional activation at enhancers and promotes megakaryocytic differentiation, providing a relevant interface to target in this poor-prognosis pediatric leukemia. PMID: 28292442
3. Clinical courses of pediatric patients with AMKL harboring the CBFA2T3-GLIS2 fusion gene are poor due to resistance to chemotherapies and SCT. New treatment strategies are necessary. PMID: 27094503
4. Nephronophthisis 7-related mutation C175R affects both localization and function of GLIS2 protein. PMID: 26374130
5. Glis2/NPHP7 is extensively modified by post-translational modifications PMID: 26083374
6. Findings are unprecedented and indicate that the DHH-RHEBL1 fusion transcript is a novel recurrent feature in the changing landscape of CBFA2T3-GLIS2-positive childhood AML. PMID: 24127550
7. BBS11 promotes accumulation of NPHP7, changing the properties of NPHP7. TRIM32 Modulates the Transcriptional Activities of Glis2 PMID: 24500717
8. Recessive mutation in GLIS2 gene is associated with Caroli disease. PMID: 23559409
9. Identification of a cryptic chromosome 16 inversion (inv(16)(p13.3q24.3)) in 27% of pediatric acute megakaryoblastic leukemia cases, which encodes a CBFA2T3-GLIS2 fusion protein. PMID: 23153540
10. Glis2 can function as a transcriptional activator and that post-translational modification within its DNA-binding domain can regulate its transcriptional activity PMID: 21127075
11. Characterization of Glis2, a novel gene encoding a Gli-related, Kruppel-like transcription factor with transactivation and repressor functions PMID: 11741991
12. Glis2 functions as a negative modulator of beta-catenin/TCF-mediated transcription. PMID: 17289029
13. identification of Gli-similar 2 (Glis2) as a novel binding protein for p120 catenin PMID: 17344476
14. Study identified Glis2 as a transcription factor mutated in Nephronophthisis and demonstrate its essential role for the maintenance of renal tissue architecture through prevention of apoptosis and fibrosis. PMID: 17618285
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相關疾病：

Nephronophthisis 7 (NPHP7)
亞細胞定位：

Nucleus speckle. Cytoplasm.
蛋白家族：

GLI C2H2-type zinc-finger protein family
組織特異性：

Expressed at high levels in kidney and at low levels in heart, lung and placenta. Expressed in colon.
數據庫鏈接：

HGNC: 29450

OMIM: 608539

KEGG: hsa:84662

STRING: 9606.ENSP00000262366

UniGene: Hs.592087

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