GAK Antibody

說明書

中文名稱：

GAK兔多克隆抗體
貨號：

CSB-PA002632
規格：

￥1090
圖片：
Western Blot analysis of 293 cells using GAK Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

O14976
基因名：

GAK
別名：

Auxilin 2 antibody; Cyclin G associated kinase antibody; Cyclin-G-associated kinase antibody; DNAJ26 antibody; DNAJC26 antibody; EC 2.7.11.1 antibody; FLJ16629 antibody; FLJ40395 antibody; GAK antibody; GAK protein antibody; GAK_HUMAN antibody; MGC99654 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from the N-terminal region of Human GAK.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:5000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
基因功能參考文獻：
1. These findings indicate that the abnormal mitoses seen after silencing OIP5-AS1 were caused by an untimely rise in GAK levels and suggest that OIP5-AS1 suppresses cell proliferation at least in part by reducing GAK levels. PMID: 28472763
2. that the multiple single nucleotide polymorphisms of GAK synergistically participate in the pathogenesis of sporadic Parkinson's disease through multiple pathways. PMID: 26676575
3. We have demonstrated that siRNA-mediated GAK silencing causes marked cell cycle disruption resulting in a raised sub-G1 alongside decreased G1 and G2/M cell cycle phases. Dual FBXW7-GAK inhibition increases multipolar mitoses PMID: 28829765
4. GAK gene expression is increased in Parkinson disease PMID: 27508417
5. These results suggest that the c-Src_GAK_MCM axis plays an important role in cell cycle progression through control of the DNA replication licensing system. PMID: 28135906
6. We filtered four OSCC genes including SERPINB9, SERPINE2, GAK, and HSP90B1 through the gene global prioritization score (P < 0.005). PMID: 26318431
7. This meta-analysis suggests that GAK rs1564282 C/T polymorphism is associated with increased susceptibility to Parkinson's disease--{REVIEW} PMID: 25975492
8. Data indicated that GBA and TMEM175/GAK significantly alter age at onset in PD. PMID: 25914293
9. AAK1 and GAK are critical regulators of HCV entry that function in part by activating EGFR, AP2M1, and NUMB, and as the molecular targets underlying the antiviral effect of sunitinib and erlotinib, respectively. PMID: 25653444
10. The effects of rs11248051 and rs1564282 variants of GAK, and the rs3129882 variant of HLA-DRA, were investigated in Parkinson's disease patients. PMID: 24039160
11. GAK apo structure reveals a dimeric inactive state of the catalytic domain mediated by an unusual activation segment interaction. PMID: 24438162
12. Cyclin-G-associated kinase is a binding partners of LRRK2, a candidate genes for risk for sporadic Parkinson disease, and part of a complex that promotes clearance of Golgi-derived vesicles through the autophagy-lysosome system. PMID: 24510904
13. Dominant negative mutants of IRAK4 and GAK show strong apoptotic effects in A498 cells under anoxia. PMID: 23591012
14. neither the CT, TT genotypes nor the minor allele T of single nucleotide polymorphism rs1564282 were associated with Parkinson's disease among the subjects from Taiwan and Singapore. PMID: 23826309
15. rs11248060 significantly increasThis study found that the minor alleles of GAK rpopulationss1564282 and DGKQ e the risk of PD in Han Chinese . PMID: 23618683
16. GAK as a regulator of dephosphorylation events under the control of the PP2A B'gamma subunit. PMID: 22262175
17. study demonstrated that the rs1564282 variant in GAK (PARK17) increases the risk of Parkinson's disease in Han Chinese patients from mainland China PMID: 22198721
18. cyclin G associated kinase is associated with Parkinson disease risk and suggest that cyclin G associated kinase and alpha-synuclein interact in a pathway involved in disease pathogenesis PMID: 21258085
19. GAK is a new candidate for investigation in future studies. PMID: 21058943
20. Osteosarcoma cell proliferation and survival are dependent on GAK. PMID: 20881269
21. results identify a role for GAK and clathrin in microtubule outgrowth from kinetochores/chromosomes and suggest that GAK acts through clathrin to control microtubule outgrowth around chromosomes PMID: 20237935
22. The GAK localizes in both cytoplasm and nucleus by immunostaining, ectopic expression of GFP-GAK and pull-down assays using dissected GAK fragments. PMID: 19371378
23. down-regulation of GAK results in outgrowth of cells in soft agar, raising the possibility that loss of GAK function may promote tumorigenesis. PMID: 15240878
24. GAK enhanced the androgen receptor transcriptional response even at low concentrations of androgens PMID: 16161052
25. Results describe the recruitment dynamics of GAK and auxilin to clathrin-coated pits during endocytosis. PMID: 16895969
26. Interactions between the PsiG[PDE][PsiLM]-motif sequences in GAK and the AP1-gamma-ear domain are critical for the recruitment of GAK to the trans-Golgi network and the function of GAK in lysosomal enzyme sorting. PMID: 17538018
27. GAK and CHC cooperated in the same pathway and interacted in mitosis to regulate the formation of a functional spindle. PMID: 19654208
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亞細胞定位：

Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network. Cell junction, focal adhesion. Note=Localizes to the perinuclear area and to the trans-Golgi network. Also seen on the plasma membrane, probably at focal adhesions.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family
組織特異性：

Ubiquitous. Highest in testis.
數據庫鏈接：

HGNC: 4113

OMIM: 602052

KEGG: hsa:2580

STRING: 9606.ENSP00000314499

UniGene: Hs.369607

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