GABARAPL2 Antibody

中文名稱：

GABARAPL2兔多克隆抗體
貨號：

CSB-PA03484A0Rb
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Raji whole cell lysate, Rat spleen tissue
All lanes: GABARAPL2 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa

IHC image of CSB-PA03484A0Rb diluted at 1:1200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA03484A0Rb diluted at 1:1200 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) GABARAPL2 Polyclonal antibody
Uniprot No.：

P60520
基因名：

GABARAPL2
別名：

ATG8 antibody; ATG8C antibody; FLC3A antibody; GABA(A) receptor-associated protein-like 2 antibody; Gabarapl2 antibody; Gamma-aminobutyric acid receptor-associated protein-like 2 antibody; Ganglioside expression factor 2 antibody; GATE-16 antibody; GATE16 antibody; GBRL2_HUMAN antibody; GEF-2 antibody; GEF2 antibody; General protein transport factor p16 antibody; Golgi-associated ATPase enhancer of 16 kDa antibody; MAP1 light chain 3-related protein antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human Gamma-aminobutyric acid receptor-associated protein-like 2 protein (1-117AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，GABARAPL2 Antibody (CSB-PA03484A0Rb)，的標記方式是Non-conjugated。對于GABARAPL2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA03484B0Rb	GABARAPL2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA03484C0Rb	GABARAPL2 Antibody, FITC conjugated
Biotin	CSB-PA03484D0Rb	GABARAPL2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:1000-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Ubiquitin-like modifier involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
基因功能參考文獻：
1. This study reveals the important role of SERP1/Ysy6 and Atg8 in endoplasmic reticulum stress response and virulence in Candida albicans. PMID: 29544880
2. Mechanism of cargo-directed Atg8 conjugation during selective autophagy has been described. PMID: 27879200
3. rs12599322 in ATG8 was significantly associated with higher DNA damage levels in Chinese population. PMID: 28512061
4. describe a protocol for the reconstituted conjugation systems for mammalian Atg8 homologs in vitro using purified recombinant Atg proteins and liposomes. PMID: 25181299
5. Using X-ray crystallography and NMR spectroscopy, the structural transition centered on the C-terminus of GATE-16 protein was identified. PMID: 26284781
6. Results showed that the mRNA and protein levels of GABARAPL1 and GATE-16 were decreased in the cerebellum of multiple system atrophy relative to controls PMID: 22959883
7. Lipidation of the LC3/GABARAP family of autophagy proteins relies on a membrane-curvature-sensing domain in Atg3. PMID: 24747438
8. these results suggest that TRPML3 plays a role in autophagosome maturation through the interaction with GATE16, by providing Ca(2+) in the fusion process. PMID: 24269818
9. low GABARAPL2/GATE-16 expression is associated with an immature myeloid leukemic phenotype and these proteins are necessary for neutrophil differentiation of APL cells PMID: 23891751
10. TP53INP1, a tumor suppressor, interacts with LC3 and ATG8-family proteins through the LC3-interacting region (LIR) and promotes autophagy-dependent cell death. PMID: 22421968
11. ATG8 proteins act as scaffolds for assembly of the ULK complex at the phagophore PMID: 23043107
12. Legionella pneumophila could interfere with autophagy by using the bacterial effector protein RavZ to directly uncouple Atg8 proteins attached to phosphatidylethanolamine on autophagosome membranes. [RavZ] PMID: 23112293
13. Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy PMID: 22885598
14. identified 14 TBC domain-containing Rab GAPs that bind directly to ATG8 modifiers and that colocalize with LC3-positive autophagy membranes in cells PMID: 22354992
15. ORP7 negatively regulates GS28 protein stability via sequestration of GATE-16, and may mediate the effect of 25-OH on GS28 and Golgi function. PMID: 21669198
16. The GATE-16 N termini in general and specific residues needed for the fusion activity are essential for the proteins role in autophagosome biogenesis. PMID: 21497758
17. Atg4B possessed the broadest spectrum against all substrates, followed by Atg4A for ATG8 substrates PMID: 21177865
18. The results indicate the essential role of the Atg8 system in the proper development of autophagic isolation membranes in mice. PMID: 18768753
19. The autophagy-unrelated association of GFP-LC3 with protein aggregates is dependent on its interaction with p62. PMID: 18776740
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亞細胞定位：

Cytoplasmic vesicle, autophagosome. Endoplasmic reticulum membrane. Golgi apparatus.
蛋白家族：

ATG8 family
組織特異性：

Ubiquitous. Expressed at high levels in the brain, heart, prostate, ovary, spleen and skeletal muscle. Expressed at very low levels in lung, thymus and small intestine.
數據庫鏈接：

HGNC: 13291

OMIM: 607452

KEGG: hsa:11345

STRING: 9606.ENSP00000037243

UniGene: Hs.461379

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