FLII Antibody

說(shuō)明書

中文名稱：

FLII兔多克隆抗體
貨號(hào)：

CSB-PA615661LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA615661LA01HU diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA615661LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) FLII Polyclonal antibody
Uniprot No.：

Q13045
基因名：

FLII
別名：

Fli 1 antibody; FLI antibody; Fli1 antibody; Flightless 1 antibody; Flightless I (Drosophila) homolog antibody; Flightless I homolog antibody; Flightless I homolog (Drosophila) antibody; Flightless1 antibody; FlightlessI antibody; fliI antibody; FLII_HUMAN antibody; Fliih antibody; FLIL antibody; MGC39265 antibody; Protein flightless 1 homolog antibody; Protein flightless-1 homolog antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Protein flightless-1 homolog protein (577-729AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，F(xiàn)LII Antibody (CSB-PA615661LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于FLII Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA615661LB01HU	FLII Antibody, HRP conjugated	ELISA
FITC	CSB-PA615661LC01HU	FLII Antibody, FITC conjugated
Biotin	CSB-PA615661LD01HU	FLII Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development. May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.
基因功能參考文獻(xiàn)：
1. Low FLII expression is associated with lung carcinoma. PMID: 28498392
2. Data show that the ability of Ca(2+) to accentuate the activity of NLRP3 inflammasome is abrogated in Flightless-I (FliI) and leucine-rich repeat FliI-interaction protein 2 (LRRFIP2)-knockdown macrophages. PMID: 27431477
3. FLII is a component of the ChREBP transcriptional complex and negatively regulates ChREBP function in cancer cells. PMID: 24055811
4. Demonstrate an important role for Flii in the development and regulation of the epidermal barrier, which may contribute to the impaired healing and skin fragility of epidermolysis bullosa patients. PMID: 24375017
5. FLII is associated with SENP3 and the MLL1/2 complex and FLII is indispensible for H3K4 methylation and proper loading of active RNA polymerase II at this gene locus. PMID: 28344658
6. Flightless-I (Drosophila) homolog (FLII) activates TGFbeta1-mediated expression of COL1A2 gene. PMID: 25451260
7. Studies suggest that Flii enhances cutaneous squamous cell carcinoma progression by decreasing apoptosis and enhancing tumor cell invasion. PMID: 26497552
8. FLII plays a tumor-suppressive role and serves as a crucial determinant of resistance of prostate cancer to endocrine therapies. PMID: 26527749
9. These data suggest FLII as a key regulator of ERalpha-mediated transcription through its role in regulating chromatin accessibility for the binding of RNA Polymerase II and possibly other transcriptional coactivators. PMID: 24632205
10. Flii is constitutively secreted from macrophages and fibroblasts and is present in human plasma. PMID: 22718342
11. Fli-I promotes the GTP-bound active Rho-mediated relief of the autoinhibition of Daam1 and mDia1. Thus, Fli-I is a novel positive regulator of Rho-induced linear actin assembly mediated by DRFs. PMID: 20223827
12. performs an essential function in early embryonic development PMID: 11971982
13. These data suggest that Flightless-I may facilitate interaction of the p160 coactivator complex with other coactivators or coactivator complexes containing actin or actin-like proteins. PMID: 14966289
14. effect of FliI protein on actin remodelling is a vital part of cellular motility, contraction and adhesion. Exact signaling pathways and mechanisms underpinning FliI effects in wound healing are yet to be fully identified[review] PMID: 17526423
15. These findings support a novel mechanism whereby cytosolic CaMK-II influences beta-catenin dependent gene expression through Fli-I. PMID: 18588881
16. The interactions between MyD88 and multiple positive and negative regulators LRRFIP2, FLAP-1, and Fliih are highly dynamic and time-course dependent in differentially regulating/modulating NF-kappa B signal transduction. PMID: 19265123
17. These findings support the model that CISK phosphorylates FLII and activates nuclear receptor transcription and suggest a new cell survival signaling pathway mediated by PI 3-kinase and CISK. PMID: 19293151
18. Recruitment of the SWI/SNF chromatin remodeling complex to steroid hormone-regulated promoters by nuclear receptor coactivator flightless-I. PMID: 19720835
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亞細(xì)胞定位：

Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell junction, focal adhesion.
組織特異性：

Strongest expression in skeletal muscle with high expression also in the heart and lung.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 3750

OMIM: 600362

KEGG: hsa:2314

STRING: 9606.ENSP00000324573

UniGene: Hs.513984

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