FCHO1 Antibody
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中文名稱：FCHO1兔多克隆抗體
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貨號：CSB-PA008546LA01HU
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規格：￥440
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圖片：
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IHC image of CSB-PA008546LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA008546LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of MCF-7 cells with CSB-PA008546LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) FCHO1 Polyclonal antibody
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Uniprot No.：
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基因名：FCHO1
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別名：FCHO1 antibody; KIAA0290F-BAR domain only protein 1 antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human F-BAR domain only protein 1 protein (45-180AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，FCHO1 Antibody (CSB-PA008546LA01HU)，的標記方式是Non-conjugated。對于FCHO1 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, IHC, IF
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推薦稀釋比：
Application Recommended Dilution IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors.
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基因功能參考文獻：
- Fcho1/2Eps15/RAP-2 ternary complexes to facilitate conformational activation of AP-2 by the Fcho1/2 interdomain linker to promote AP-2 cargo engagement. PMID: 27237791
- The central linker of FCHO proteins acts as an allosteric regulator of the prime endocytic adaptor, AP-2. PMID: 25303365
- show that the mu-homology domain of FCHO1/2 represents an endocytic interaction hub PMID: 22484487
- study reports FCHo1/2 were required for plasma membrane clathrin-coated vesicle (CCV) budding & marked sites of CCV formation PMID: 20448150
- Involved in clathrin-coated vesicle formation. PMID: 17617719
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亞細胞定位：Membrane, clathrin-coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Associated with forming but not mature clathrin-coated vesicles. The recruitment to coated-pits precede the one of clathrin and the adaptor protein complex AP-2. According to PubMed:17617719 it may also dynamically associate with Golgi/TGN membranes.
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蛋白家族：FCHO family
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數據庫鏈接：
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