FBXO32 Antibody

說明書

中文名稱：

FBXO32兔多克隆抗體
貨號：

CSB-PA996906
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA996906(FBXO32 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA996906(FBXO32 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HT29 cells, human liver cancer tissue, Primary antibody: CSB-PA996906(FBXO32 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

Q969P5
基因名：

FBXO32
別名：

4833442G10Rik antibody; AI430017 antibody; Atrogin 1 antibody; Atrogin-1 antibody; ATROGIN1 antibody; Atrophy gene 1 antibody; F box only protein 32 antibody; F-box only protein 32 antibody; F-box protein 32 antibody; FBX32_HUMAN antibody; fbxo25 antibody; FBXO32 antibody; FLJ32424 antibody; MAFbx antibody; MGC108443 antibody; MGC137646 antibody; MGC33610 antibody; Muscle atrophy F box antibody; Muscle atrophy F box protein antibody; Muscle atrophy F-box protein antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Fusion protein of Human FBXO32
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:1000-1:5000
WB	1:200-1:1000
IHC	1:25-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
基因功能參考文獻：
1. F-box only protein 32 (FBXO32) directly ubiquitinates C-terminal binding protein 1 (CtBP1), which is required for its stability and nuclear retention. PMID: 29142217
2. Authors found that the FBXO32 and SMAD4 levels were higher in normal tissues than in CRC tissues. The expressions of FBXO32 and SMAD4 were related to clinicopathological parameters in CRC. PMID: 29465067
3. FBXO32 activates NF-kappaB through IkappaBalpha degradation in inflammatory and genotoxic stress PMID: 28970077
4. Transcriptional analysis of endophilin-A mutant mice, complemented by proteomics, highlighted ataxia- and protein-homeostasis-related genes and revealed upregulation of the E3-ubiquitin ligase FBXO32/atrogin-1 and its transcription factor FOXO3A. PMID: 27720640
5. Low FBXO32 expression is associated with breast cancer tumorigenesis. PMID: 28068319
6. Our results indicate that abnormal SCF activity with subsequent impairment of the autophagic flux due to a novel FBXO32 mutation is implicated in the pathogenesis of Dilated cardiomyopathy . PMID: 26753747
7. Our data suggest that FBXO32 is a candidate gene for recessive familial dilated cardiomyopathy. Acting as a cardiac ubiquitin ligase, mutated FBXO32 could perturb the degradation of target proteins in the ubiquitin proteasome system. PMID: 26768247
8. Atrogin-1 expression tended to be increased in the skeletal muscle of patients with malignant disease even before cancer related cachexia weight loss. PMID: 25760630
9. FBXO32 targets Lys-326 of c-Myc to form polyubiquitin chains, resulting in inhibition of cell proliferation. PMID: 25944903
10. MAFbx not only regulates protein degradation, but also reduces protein synthesis, exerting a dual role in regulating cardiac mass and preventing from cardiac hypertrophy. PMID: 24650875
11. FBXO32 methylation status and protein expression were independently associated with survival in ESCC. FBXO32 may be a functional tumor suppressor. Its inactivation through promoter methylation could play an important role in ESCC carcinogenesis. PMID: 24798237
12. EZH2 is a key factor in the proliferation and survival of PAX3-FOXO1 alveolar RMS cells working, at least in part, by repressing FBXO32 PMID: 24213577
13. ATROGIN1 gene expression is increased in patients with severe burn injury. PMID: 23816995
14. Quadriceps muscle atrogin-1 levels were lower in COPD patients than controls, but similar in patients with a low and normal fat-free mass index. Atrogin1 levels were not associated with quadriceps fiber cross-sectional area or strength in patients. PMID: 23844868
15. Data suggest expression of atrogin-1 and MuRF-1 (muscle-specific RING finger protein 1) play role in aging-related decrease in muscle mass (i.e., sarcopenia); up-regulation of atrogin-1/MuRF-1 has potential to prevent/reverse sarcopenia. [REVIEW] PMID: 22815045
16. Cardiac and vascular atrogin-1 mRNA expression is not associated with dexamethasone efficacy in the monocrotaline model of pulmonary hypertension. PMID: 22311109
17. In a transgenic mouse model of autoimmune myositis, cellular inflammation is associated with FOXO3A translocation and Atrogin-1 induction. PMID: 22590725
18. MAFbx contains two functional nuclear localization signals (NLS). Mutation or deletion of only one NLS induced cytoplasmic localization of MAFbx. PMID: 22249105
19. investigation of factors regulating expression of two ubiquitin ligases (MAFbx and MURF1) in skeletal muscle (i.e., vastus lateralis): effects of resistance exercise and anabolic dietary supplement (i.e., branched-chain amino acids) PMID: 22127230
20. SerpinB5 interacts with KHDRBS3 and FBXO32, and KHDRBS3 can interact with FBXO32 mRNA. PMID: 21725612
21. atrophic AKT-FOXO signaling play major roles in eliciting pathological changes associated with diaphragm disuse. PMID: 20833824
22. The novel tumor suppressor FBXO32 is epigenetically silenced in ovarian cancer cell lines with disrupted TGF-beta/SMAD4 signaling, and FBXO32 methylation status predicts survival in patients with ovarian cancer. PMID: 20065949
23. Human skeletal muscle atrophy in the amyotrophic lateral sclerosis shows an increase in atrogin-1 & a decrease in Akt. The transcriptional regulation of human atrogin-1 may be controlled by an Akt-mediated transcription factor other than FKHR. PMID: 16507768
24. results suggest that Cbl-b- or atrogin-1-mediated ubiquitination plays an important role in unloading-induced muscle atrophy, and that unloading stress may preferentially inhibit transcriptional responses in skeletal muscle PMID: 16868939
25. Atrogin-1 mRNA expression was significantly increased in quadriceps of patients with COPD; transcriptional regulation of atrogin-1 and MuRF1 may occur via FoxO-1, but independently of AKT PMID: 17478621
26. CSRP3, MUSTN1, SIX1, and FBXO32 expression changes in response to lengthening and shortening contractions in human muscle PMID: 17519359
27. We conclude that smoking impairs the muscle protein synthesis process and increases the expression of genes associated with impaired muscle maintenance; smoking therefore likely increases the risk of sarcopenia. PMID: 17609255
28. Results showed upregulation of MuRf1 and MAFbx in atrophies muscle and support their role as regulatory peptides in various conditions which lead to muscle atrophy. PMID: 17977773
29. atrogin-1 may be a critical mediator of the muscle damage induced by statins. PMID: 17992259
30. Testosterone represses MAFbx expression via interactions of the AR with Oct-1. PMID: 18599544
31. Review discusses findings implicating atrogin-1, a gene required for muscle atrophy, in the pathophysiology of statin-induced muscle injury. PMID: 18681786
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相關疾?。?/div>
Defects in FBXO32 are associated with susceptibility to dilated cardiomyopathy (DCM). A disorder characterized by ventricular and impaired systolic function, resulting in heart failure and arrhythmia. Patient are at risk of premature death.
亞細胞定位：

Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and the nucleus.
組織特異性：

Specifically expressed in cardiac and skeletal muscle.
數據庫鏈接：

HGNC: 16731

OMIM: 606604

KEGG: hsa:114907

STRING: 9606.ENSP00000428205

UniGene: Hs.403933

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