FBXO10 Antibody

中文名稱：

FBXO10兔多克隆抗體
貨號：

CSB-PA890915LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA890915LA01HU diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA890915LA01HU diluted at 1:500 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA890915LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: FBXO10 antibody at 4.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 106, 53 kDa
Observed band size: 106 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) FBXO10 Polyclonal antibody
Uniprot No.：

Q9UK96
基因名：

FBXO10
別名：

F box protein 10 antibody; F-box only protein 10 antibody; FBX10 antibody; FBX10_HUMAN antibody; Fbxo10 antibody; hCG_2011110 antibody; PRMT11 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human F-box only protein 10 protein (248-400AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，FBXO10 Antibody (CSB-PA890915LA01HU)，的標記方式是Non-conjugated。對于FBXO10 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA890915LB01HU	FBXO10 Antibody, HRP conjugated	ELISA
FITC	CSB-PA890915LC01HU	FBXO10 Antibody, FITC conjugated
Biotin	CSB-PA890915LD01HU	FBXO10 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. The SCF(FBXO10) complex mediates ubiquitination and degradation of BCL2, an antiapoptotic protein, thereby playing a role in apoptosis by controlling the stability of BCL2.
基因功能參考文獻：
1. siRNA-mediated knockdown of FBXO11 facilitated HIF-1alpha expression in various cancer cells and HIF-1alpha-driven gene expressions, but the FBXO10 knockdown did not. PMID: 26187670
2. Higher Fbxo10 expression in T cells is associated with Mcs5a increased susceptibility alleles PMID: 23138933
3. FBXO10, a protein related to DRE-1, binds BCL2 and promotes its degradation, thereby initiating cell death. Moreover, some diffuse large B-cell lymphomas have inactivating mutations in FBXO10 or express FBXO10 at low levels. PMID: 23431138
相關疾病：

Defects in FBXO10 may be a cause of diffuse large B-cell lymphoma by allowing the accumulation of BCL2, an oncoprotein that has a critical role in lymphomas.
亞細胞定位：

Cytoplasm.
數據庫鏈接：

HGNC: 13589

OMIM: 609092

KEGG: hsa:26267

STRING: 9606.ENSP00000403802

UniGene: Hs.709527

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