ELP3 Antibody

中文名稱：

ELP3兔多克隆抗體
貨號：

CSB-PA884499LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate, K562 whole cell lysate, 293 whole cell lysate
All lanes: ELP3 antibody at 3.82µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63, 61, 50, 53 kDa
Observed band size: 63 kDa

IHC image of CSB-PA884499LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA884499LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating ELP3 in HEK293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA884499LA01HU in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA884499LA01HU (6µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (20µg)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ELP3 Polyclonal antibody
Uniprot No.：

Q9H9T3
基因名：

ELP3
別名：

DKFZp761L098 antibody; Elongation protein 3 homolog (S. cerevisiae) antibody; Elongation protein 3 homolog antibody; ELONGATION PROTEIN 3; S. CEREVISIAE; HOMOLOG OF antibody; elongator acetyltransferase complex subunit 3 antibody; Elongator complex protein 3 antibody; elp3 antibody; ELP3_HUMAN antibody; FLJ10422 antibody; hELP3 antibody; Hypothetical protein DKFZp761L098 antibody; Kat9 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Elongator complex protein 3 protein (1-85AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ELP3 Antibody (CSB-PA884499LA01HU)，的標記方式是Non-conjugated。對于ELP3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA884499LB01HU	ELP3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA884499LC01HU	ELP3 Antibody, FITC conjugated
Biotin	CSB-PA884499LD01HU	ELP3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex.
基因功能參考文獻：
1. and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
2. Promoter hypermethylation is an important mechanism of the transcriptional inactivation of ELP3 in invasive ductal breast carcinoma. PMID: 25148870
3. Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress. PMID: 24769394
4. data suggest that hElp3 can regulate the transcription of HSP70 gene, and the HAT domain of hElp3 is essential for this function PMID: 22216241
5. The results of this study uncover a novel role for Elp3 in the regulation of synaptic bouton expansion during neurogenesis that may be linked with a requirement for sleep. PMID: 20626565
6. Elp3 promotes the acetylation of alpha-tubulin in microtubules in neurological disorders. PMID: 20036197
7. We used hELP3 antisense oligonucl. to knock down hELP3 gene. The results showed that reduction of hELP3 mRNA and protein caused a suppression of HSP70-2 and histone H3 hypoacetylation. PMID: 17558451
8. Allelic variants of ELP3 were associated with amyotrophic lateral sclerosis in three populations comprising 1483 people. PMID: 18996918
9. ELP3 localises to mitochondria in HeLa cells, actin-like filaments, and actin-rich sites at the edges of spreading cells. This suggests that ELP3 and the ELONGATOR complex may play a role in mitochondrial function, actin organisation, and cell motility. PMID: 19429107
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相關疾病：

ELP3 genetic variations may be associated with an increased risk for neurodegeneration and motor neuron diseases.
亞細胞定位：

Cytoplasm. Nucleus.; [Isoform 1]: Nucleus.; [Isoform 2]: Cytoplasm. Nucleus.
蛋白家族：

ELP3 family
組織特異性：

Expressed in the cerebellum and spinal motor neurons.
數據庫鏈接：

HGNC: 20696

OMIM: 612722

KEGG: hsa:55140

STRING: 9606.ENSP00000256398

UniGene: Hs.491336

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