EIF4G2 Antibody

說明書

中文名稱：

EIF4G2兔多克隆抗體
貨號：

CSB-PA002071
規格：

￥1090
圖片：
Western Blot analysis of A549 cells using DAP-5 Polyclonal Antibody

Western Blot analysis of HepG2 cells using DAP-5 Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P78344
基因名：

EIF4G2
別名：

DAP-5 antibody; DAP5 antibody; Death associated protein 5 antibody; Death-associated protein 5 antibody; eIF-4-gamma 2 antibody; eIF-4G 2 antibody; eIF4G 2 antibody; EIF4G2 antibody; Eukaryotic translation initiation factor 4 gamma 2 antibody; IF4G2_HUMAN antibody; Nat1 antibody; Novel APOBEC-1 target 1 antibody; p97 antibody; Translation repressor NAT1 antibody; Translation repressor NAT1 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from the N-terminal region of Human DAP-5.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.
基因功能參考文獻：
1. The percentage at the S-stage was decreased in the miR-379 mimics and EIF4G2 shRNA groups. PMID: 28381518
2. DAP5 knockdown from human ESCs (hESCs) resulted in persistence of pluripotent gene expression, delayed induction of differentiation-associated genes in different cell lineages, and defective embryoid body formation PMID: 27664238
3. Data show that microRNA miR-379 potentiated lung cancer (LCa) chemosensitivity via modulation of cisplatin (CDDP)-induced apoptosis by directly targeting the eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) 3' UTR. PMID: 28117895
4. These results indicate that miR-139 is capable of inhibiting chondrocyte proliferation and migration, thus being a possible therapeutic target for OA. The mechanism of miR-139 in chondrocytes may be related to its regulation on EIF4G2 and IGF1R. PMID: 27105918
5. The Coxsackievirus B3 protease 2A-mediated cleavage of DAP5 results in the production of two truncates that exert differential effects on protein translation of the IRES-containing genes, leading to enhanced host cell death. PMID: 26586572
6. Knockdown of EIF4G2 recapitulated the effects of mir-139, whereas restoring EIF4G2 expression rescued the mir-139 phenotype. elevated miR-139-5p expression is associated with a favorable outcome in acute myeloid leukemia. PMID: 26165837
7. findings provide the first mechanistic insights into the function of DAP5 as a selective regulator of cap-independent translation PMID: 25779044
8. Our results provide evidence that the tumor suppressor effect of miR-520c-3p is mediated through repression of translation while inducing senescence and that eIF4GII is a key effector of this anti-tumor activity. PMID: 24497838
9. DAP5, a translation initiation factor shown to positively regulate the translation of various internal ribosome entry sites containing mRNAs, promotes internal ribosome entry site-driven translation of p53 mRNA. PMID: 23318444
10. DAP5/p97 and DAP5/p86 enhanced the translation of the anti-apoptotic protein Bcl-2 and inhibited cisplatin-induced apoptosis PMID: 22555068
11. Multiple isoforms of eIF4GII arise from multiple promoters & alternative splicing events. A non-canonical CUG initiation codon extends the eIF4GII N-terminus. The eIF4GII N-terminus plays an alternative role in initiation factor assembly. PMID: 22909319
12. crystal structure of C-terminal region (residues 540-897) of p97/EIF4G2 (at 2.0 Angstrom resolution): sequence/structure homology with EIF4G1 PMID: 20544972
13. crystallization and preliminary X-ray diffraction analysis of the MIF4G domain of DAP5 is presented. PMID: 20057060
14. Includes the functional analysis of a similar protein in yeast. PMID: 11331597
15. The caspase-cleaved DAP5 protein supports internal ribosome entry site-mediated translation of death proteins PMID: 11943866
16. endogenous eIF4GII was phosphorylated by Ca(2+)/calmodulin-dependent protein kinase I PMID: 14507913
17. Cleavage of eIF4GII is induced in cells and in cell extracts by the FMDV leader protease (L(pro)) alone PMID: 15016848
18. EIF4G2 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed and EIF4G2 gene transcription was associated with invasive tumors. PMID: 16410684
19. p97 is functionally different from the closely related C-terminal two-thirds of eIF4GI and it can positively promote protein synthesis and cell proliferation. PMID: 16932749
20. These data indicate that full-length p97 is a translational activator with essential role(s) in unstressed cells, suggesting a reassessment of current models of p97 function. PMID: 17237356
21. Data show that DAP5 promotes cap-independent translation of Bcl-2 and CDK1 to facilitate cell survival during mitosis. PMID: 18450493
22. role and regulation of death-associated protein-5 (DAP5/p97/NAT1), a novel inhibitor of translational initiation, in acute promyelocytic leukemia cell differentiation and apoptosis PMID: 18491231
23. Results report the crystal structure of the C-terminal region of DAP5/p97 extending between amino acids 730 and 897. PMID: 18722383
24. Exclusive use of a GUG translation initiation codon is conserved in human, chicken, Xenopus, and zebrafish. PMID: 15718103
25. translation starts exclusively at a non-AUG translation initiation codon (GUG). PMID: 9049310
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蛋白家族：

Eukaryotic initiation factor 4G family
組織特異性：

Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.
數據庫鏈接：

HGNC: 3297

OMIM: 602325

KEGG: hsa:1982

STRING: 9606.ENSP00000340281

UniGene: Hs.183684

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