EIF3G Antibody

文獻引用 (1) 說明書

中文名稱：

EIF3G兔多克隆抗體
貨號：

CSB-PA03099A0Rb
規格：

￥440
圖片：
IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with CSB-PA03099A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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產品評價
引用文獻
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) EIF3G Polyclonal antibody
Uniprot No.：

O75821
基因名：

EIF3G
別名：

44kDa antibody; Anti EIF3S4 antibody; eIF 3 delta antibody; eIF 3 RNA binding subunit antibody; eIF-3 RNA-binding subunit antibody; eIF-3-delta antibody; eIF3 delta antibody; eIF3 p42 antibody; eIF3 p44 antibody; eIF3g antibody; EIF3G_HUMAN antibody; EIF3S4 antibody; EIF3S4 antibody antibody; EIF3S4; formerly antibody; Eukaryotic translation initiation factor 3 antibody; Eukaryotic translation initiation factor 3 RNA binding subunit antibody; Eukaryotic translation initiation factor 3 RNA-binding subunit antibody; Eukaryotic translation initiation factor 3 subunit 4 antibody; Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody; Eukaryotic translation initiation factor 3 subunit G antibody; Eukaryotic translation initiation factor 3 subunit p42 antibody; Eukaryotic translation initiation factor 3; subunit 4; formerly antibody; Subunit 4 (delta 44kD) antibody; Subunit 4 delta antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Eukaryotic translation initiation factor 3 subunit G protein (2-320AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，EIF3G Antibody (CSB-PA03099A0Rb)，的標記方式是Non-conjugated。對于EIF3G Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA03099B0Rb	EIF3G Antibody, HRP conjugated	ELISA
FITC	CSB-PA03099C0Rb	EIF3G Antibody, FITC conjugated
Biotin	CSB-PA03099D0Rb	EIF3G Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection Garcia-Moreno M, et al,Molecular Cell,2019

產品評價

靶點詳情

功能：

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.; (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.
基因功能參考文獻：
1. The disease-associated allele increases EIF3G mRNA expression. EIF3G is located in the narcolepsy risk locus and EIF3G expression correlates with PPAN and P2RY11 expression. PMID: 25669430
2. Important roles for eIF3g in the translation initiation machinery and in DNA degradation during apoptosis. PMID: 24080033
3. down-regulation of eIF3g inhibits the efficiency of nonsense-mediated mRNA decay, which is tightly coupled to CT but not to ET PMID: 22493286
4. Furthermore, we found that overexpression of DISC1 in SH-SY5Y cells induces the assembly of eIF3- and TIA-1-positive stress granules (SGs), discrete cytoplasmic granules formed in response to environmental stresses. PMID: 16243297
5. AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein PMID: 17094969
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region.
蛋白家族：

EIF-3 subunit G family
數據庫鏈接：

HGNC: 3274

OMIM: 603913

KEGG: hsa:8666

STRING: 9606.ENSP00000253108

UniGene: Hs.529059

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