EIF3F Antibody

說明書

中文名稱：

EIF3F兔多克隆抗體
貨號：

CSB-PA975347
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA975347(EIF3F Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA975347(EIF3F Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse skin tissue, Primary antibody: CSB-PA975347(EIF3F Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds
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靶點詳情

產品詳情

Uniprot No.：

O00303
基因名：

EIF3F
別名：

Deubiquitinating enzyme eIF3f antibody; eIF 3 epsilon antibody; eIF-3-epsilon antibody; eIF3 p47 antibody; eIF3 p47 subunit antibody; eIF3-epsilon antibody; eIF3-p47 antibody; eIF3f antibody; EIF3F_HUMAN antibody; EIF3S5 antibody; EIF3S5; formerly antibody; Eukaryotic translation initiation factor 3 subunit 5 antibody; Eukaryotic translation initiation factor 3 subunit 5; formerly antibody; Eukaryotic translation initiation factor 3 subunit F antibody; eukaryotic translation initiation factor 3; subunit 5 (epsilon; 47kD) antibody; eukaryotic translation initiation factor 3; subunit 5 epsilon; 47kDa antibody; eukaryotic translation initiation factor 3; subunit F antibody; translation initiation factor 3 47 kda subunit antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Fusion protein of Human EIF3F
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
WB	1:200-1:1000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.; Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.
基因功能參考文獻：
1. The overexpression of eIF3f suppressed Akt and ERK signaling and subsequently depleted CLU expression. In addition, eIF3F stabilized p53, which increased the expression of p21 and Bax. PMID: 26988917
2. eIF3f/alpha adrenergic receptor interaction PMID: 26497985
3. Decreased expression of eukaryotic initiation factor 3f is associated with gastric cancer. PMID: 24678890
4. eIF3f may play an important role in recurrence. PMID: 24732644
5. findings established a new mechanism of rRNA decay regulation mediated by hnRNP K/eIF3f and suggest that the tumor suppressive function of eIF3f may link to impaired rRNA degradation and translation PMID: 22457825
6. catalytically inactive forms of eIF3f as well as shRNAs targeting eIF3f repress Notch activation in a coculture assay, showing that eIF3f is a new positive regulator of the Notch pathway PMID: 21124883
7. Loss of EIF3F in pancreatic cancer is reported. PMID: 17918192
8. demonstrated a statistically significant decrease of the eIF3f gene copy number in melanoma compared with normal tissues with a tumor/normal ratio of 0.52 PMID: 18381585
9. eIF3f specifically interferes with the 3' end processing of HIV-1 mRNAs PMID: 19237569
10. eIF3f may inhibit translation by increasing the binding to the eIF3 complex during apoptosis. PMID: 19245811
11. eIF3f mediates restriction of HIV-1 expression through a set of factors that includes eIF3f, the SR protein 9G8, and the cyclin-dependent kinase 11 (CDK11). PMID: 19854136
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亞細胞定位：

Cytoplasm.
蛋白家族：

EIF-3 subunit F family
數據庫鏈接：

HGNC: 3275

OMIM: 603914

KEGG: hsa:8665

STRING: 9606.ENSP00000310040

UniGene: Hs.516023

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