EFHD1 Antibody
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中文名稱(chēng)：EFHD1兔多克隆抗體
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貨號(hào)：CSB-PA880107LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate
All lanes: EFHD1 antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 27, 17 kDa
Observed band size: 27 kDa -
IHC image of CSB-PA880107LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of A549 cells with CSB-PA880107LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱(chēng)：Rabbit anti-Homo sapiens (Human) EFHD1 Polyclonal antibody
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Uniprot No.：
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基因名：EFHD1
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別名：4931430I01Rik antibody; AI452351 antibody; DKFZp781H0842; antibody; EF hand domain containing 1 antibody; EF hand domain family; member D1 antibody; EF-hand domain-containing protein 1 antibody; EF-hand domain-containing protein D1 antibody; EFHD1 antibody; EFHD1_HUMAN antibody; FLJ13612 antibody; MGC103094 antibody; Mitocalcin antibody; MST133 antibody; MSTP133 antibody; PP3051 antibody; RGD1559565 antibody; Swiprosin 2 antibody; Swiprosin-2 antibody; SWS2 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human EF-hand domain-containing protein D1 protein (15-72AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁(yè)面中的產(chǎn)品，EFHD1 Antibody (CSB-PA880107LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于EFHD1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：
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克隆類(lèi)型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:500-1:1000 IF 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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靶點(diǎn)詳情
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功能：Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production. May play a role in neuronal differentiation.
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基因功能參考文獻(xiàn)：
- EFHD1 functions as a novel mitochondrial Ca(2+) sensor underlying Ca(2+)-dependent activation of mitoflashes PMID: 26975899
- detection of methylation of PPP1R3C alone or in combination with EFHD1 in plasma DNA showed high sensitivity and specificity in CRC detection, and may be useful detection method for CRC, especially for early-stage CRCs. PMID: 24861485
- Cloning of novel mouse genes associated with neuronal function, including Efhd1. PMID: 12270117
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亞細(xì)胞定位：Mitochondrion inner membrane.
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數(shù)據(jù)庫(kù)鏈接：
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