EEA1 Antibody

中文名稱：

EEA1兔多克隆抗體
貨號：

CSB-PA007405LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate
All lanes: EEA1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 163 kDa
Observed band size: 163 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007405LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA007405LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA007405LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) EEA1 Polyclonal antibody
Uniprot No.：

Q15075
基因名：

EEA1
別名：

Early endosome antigen 1 antibody; Early endosome antigen 1; 162kD antibody; Early endosome associated protein antibody; EEA 1 antibody; EEA1 antibody; EEA1_HUMAN antibody; Endosome associated protein p162 antibody; Endosome-associated protein p162 antibody; MST105 antibody; MSTP105 antibody; ZFYVE2 antibody; Zinc finger FYVE domain containing protein 2 antibody; Zinc finger FYVE domain-containing protein 2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Early endosome antigen 1 protein (734-875AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，EEA1 Antibody (CSB-PA007405LA01HU)，的標記方式是Non-conjugated。對于EEA1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA007405LB01HU	EEA1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007405LC01HU	EEA1 Antibody, FITC conjugated
Biotin	CSB-PA007405LD01HU	EEA1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
基因功能參考文獻：
1. This also suggests that this pathway is involved in EEA1-vesicles biogenesis. PMID: 30188634
2. identification of allergic bronchopulmonary aspergillosis -associated variants in EEA that have functional effects on macrophage phagocytosis and phagolysosome acidification of A. fumigatus conidia. PMID: 29547649
3. results suggest a new mechanism in which Rab5 induces a change in flexibility of EEA1, generating an entropic collapse force that pulls the captured vesicle towards the target membrane to initiate docking and membrane fusion PMID: 27556945
4. EEA1 mobility on endosomes is decreased upon stimulation of EGF receptor endocytosis in HeLa cells PMID: 26993163
5. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized TbetaRI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). PMID: 25998683
6. In serum-deprivated HeLa cells with low endocytic activity there two types of EEA1-vesicles: the first one carries the both EEA1 and Rab5 at high levels; the second consists of weakly decorated EEA1-vesicles that can be both Rab5-positive and -negative. PMID: 25711083
7. Data indicate that early endosome antigen 1 (EEA1) is important for the small GTPase Rab31-mediated enhancement of ligand-bound EGF receptor (EGFR) endocytic trafficking. PMID: 24644286
8. nuclear uptake of Abeta involves the dynamin-dependent EEA1 and TGF-beta/Smad signaling pathways. PMID: 24491918
9. We therefore propose that the N1072K variant of the EEA1 gene is a candidate mutation for susceptibility to diabetes in the Japanese population. PMID: 23499280
10. EEA1 interacts with Phafin2 and colocolizes with Phafin2 in endosome membrane. PMID: 22816767
11. NMR analyses of the interaction between the FYVE domain of early endosome antigen 1 (EEA1) and phosphoinositide embedded in a lipid bilayer. PMID: 22915584
12. p97 inhibition causes increased EEA1 self-association at the endosome membrane. PMID: 21556036
13. The knockdown of STEAP4 inhibits insulin-stimulated glucose transport and GLUT4 translocation via the attenuated phosphorylation of Akt, independent of the effects of EEA1. PMID: 21468601
14. crystal structure of Rab5A in complex with the EEA1 C(2)H(2) zinc finger PMID: 20534488
15. activation of p38 MAPK caused a decrease in EEA1 colocalization with phagosomes, arresting maturation into the phagolysosome PMID: 12963735
16. macropinosomes do not mature to late endosomes or fuse with lysosomes. EEA1 continuously mediates homotypic fusion as long as the macropinosomes persist. PMID: 15052657
17. These results suggest that EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25. PMID: 15451443
18. Results describe the geometry of the micelle penetration of the early endosome antigen 1 FYVE domain. PMID: 16331966
19. PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs PMID: 16448788
20. Impairment of internalization of specific glutamate receptors and their subsequent accumulation in the synapse may account for the neurological deficits observed in some patients developing EEA1 autoantibodies. PMID: 17113235
21. results suggest that the local production of PtdIns(3)P implicates the fusion of macropinosomes via EEA1 as well as conventional early endosomes PMID: 17146146
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亞細胞定位：

Cytoplasm. Early endosome membrane; Peripheral membrane protein.
數據庫鏈接：

HGNC: 3185

OMIM: 605070

KEGG: hsa:8411

STRING: 9606.ENSP00000317955

UniGene: Hs.567367

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