DPPA5 Antibody
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中文名稱：DPPA5兔多克隆抗體
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貨號：CSB-PA007147LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: DPPA5 antibody at 4.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa -
IHC image of CSB-PA007147LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA007147LA01HU diluted at 1:200 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA007147LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) DPPA5 Polyclonal antibody
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Uniprot No.：
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基因名：DPPA5
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別名：Developmental pluripotency associated 5 antibody; Developmental pluripotency-associated 5 protein antibody; DPPA5 antibody; DPPA5_HUMAN antibody; Embryonal stem cell specific gene 1 protein antibody; Embryonal stem cell-specific gene 1 protein antibody; Esg 1 antibody; Esg-1 antibody; ESG1 antibody; hDPPA5 antibody
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宿主：Rabbit
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反應(yīng)種屬：Human, Mouse
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免疫原：Recombinant Human Developmental pluripotency-associated 5 protein (1-116AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁面中的產(chǎn)品，DPPA5 Antibody (CSB-PA007147LA01HU)，的標(biāo)記方式是Non-conjugated。對于DPPA5 Antibody，我們還提供其他標(biāo)記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs.
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基因功能參考文獻(xiàn)：
- Our study provides new insight into the function of DPPA5 and NANOG regulation in human pluripotent stem cell . PMID: 26661329
- Dppa5 is specifically and differentially expressed in human cells that have pluripotency and can be used as a marker of pluripotent stem cells. PMID: 15790765
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亞細(xì)胞定位：Cytoplasm.
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蛋白家族：KHDC1 family
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數(shù)據(jù)庫鏈接：
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