DPH5 Antibody

中文名稱：

DPH5兔多克隆抗體
貨號(hào)：

CSB-PA862001LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA862001LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of SH-SY5Y cells with CSB-PA862001LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DPH5 Polyclonal antibody
Uniprot No.：

Q9H2P9
基因名：

DPH5
別名：

DPH5 antibody; AD-018 antibody; CGI-30 antibody; HSPC143 antibody; NPD015Diphthine methyl ester synthase antibody; EC 2.1.1.314 antibody; Diphthamide biosynthesis methyltransferase antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Diphthine methyl ester synthase protein (138-235AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，DPH5 Antibody (CSB-PA862001LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DPH5 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA862001LB01HU	DPH5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA862001LC01HU	DPH5 Antibody, FITC conjugated
Biotin	CSB-PA862001LD01HU	DPH5 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.
基因功能參考文獻(xiàn)：
1. The yeast and Chinese hamster homologs of human DPH5 catalyze the trimethylation step in the biosynthesis of diphthamide PMID: 15485916
蛋白家族：

Diphthine synthase family
數(shù)據(jù)庫鏈接：

HGNC: 24270

OMIM: 611075

KEGG: hsa:51611

STRING: 9606.ENSP00000359127

UniGene: Hs.440776

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