DNAJB1 Antibody

說明書

中文名稱：

DNAJB1兔多克隆抗體
貨號：

CSB-PA288820
規格：

￥2024
圖片：
Western blot analysis of extracts from COLO205 cells, using HSP40 antibody.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HSP40 antibody.

Immunofluorescence analysis of NIH/3T3 cells, using HSP40 antibody.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DNAJB1 Polyclonal antibody
Uniprot No.：

P25685
基因名：

DNAJB1
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human HSP40.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:3000
IHC	1:50-1:100
IF	1:100-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response. Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro).
基因功能參考文獻：
1. this study shows that DNAJB1/HSP40 suppresses Melanoma Differentiation-Associated Gene 5-Mitochondrial Antiviral Signaling Protein function in conjunction with HSP70 PMID: 29069650
2. Modeling of the different conformations of PRKACA-DNAJB1 Chimeric Kinase revealed no obvious steric interactions of the J-domain with the rest of the RIIbeta holoenzyme. PMID: 29335433
3. Data show that DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1) is a transcriptional target of forkhead box protein E3 (FOXE3) in a pathway that is crucial for the development of the anterior segment of the eye. PMID: 27218149
4. These results indicate that Mixed fibrolamellar hepatocellular carcinoma (mFL-HCC) is similar to pure FL-HCC at the genomic level and the DNAJB1:PRKACA fusion can be used as a diagnostic tool for both pure and mFL-HCC PMID: 27029710
5. These studies demonstrate an important role for cellular chaperone Hsp40/DnaJB1 in influenza A virus life cycle by assisting nuclear trafficking of viral ribonucleoproteins. PMID: 26750153
6. DNAJB1-PRKACA was evaluated as a key driver of fibrolamellar carcinoma and as a candidate therapeutic target PMID: 26505878
7. study provides a new molecular mechanism to regulate EGFR signaling through modulation of MIG6 by DNAJB1 as a negative regulator. PMID: 26239118
8. Whole exome sequencing followed by immunohistochemistry of fibrolamellar hepatocellular carcinoma cell lines and tumors showed two structural variants resulting in fusion transcripts: DNAJB1-PRKCA and CLPTM1L-GLIS3. PMID: 25122662
9. In this cohort of fibrolamellar hepatocellular carcinoma, almost 80% contained the DNAJB1-PRKACA fusion transcript. PMID: 25557953
10. Authors identified DNAJB1 as a negative regulator of PDCD5-mediated apoptosis and found that the apoptosis network of PDCD5 regulates cancer cell death. PMID: 25444898
11. We also found that, despite an initial report to the contrary, the human homolog of Sis1, Hdj1, is capable of [PSI+] prion propagation in place of Sis1 PMID: 25058638
12. HSP40 interacts with pyruvate kinase M2 and regulates glycolysis and cell proliferation in tumor cells. PMID: 24658033
13. Studies with specific phosphoantibodies indicate that MK5 phosphorylates Hsp40/DnaJB1 in vivo at Ser-149 or/and Ser-151 and Ser-171 in the C-terminal domain of Hsp40/DnaJB1. PMID: 24309468
14. Evidence supporting the presence of the DNAJB1-PRKACA chimeric transcript in 100% of the FL-HCCs examined (15/15) suggests that this genetic alteration contributes to tumor pathogenesis. PMID: 24578576
15. we discovered a new MDM2 interacting protein, DNAJB1, and provided evidence to support its p53-dependent tumor suppressor function. PMID: 24361594
16. HSP40 and HSP110 function together in protein homeostasis control. PMID: 24091676
17. methionine deprivation results in an antioxidant response that includes an increase in the levels of HSPA1A and DNAJB1 mRNA PMID: 23395854
18. There was a positive correlation between DnaJB1 and severity of pulmonary arterial hypertension in peripheral blood mononuclear cells from patients with limited cutaneous systemic sclerosis. PMID: 23400395
19. Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway. PMID: 22075554
20. Mammalian Hsp110 (Apg-2), Hsp70 (Hsc70 or Hsp70) and Hsp40 (Hdj1) were necessary and sufficient to slowly dissolve large disordered aggregates and recover natively folded protein PMID: 22022600
21. Cellular localization studies showed that NP and Hsp40 co-localize primarily in the nucleus. During IAV infection in mammalian cells, expression of NP coincided with the dissociation of P58(IPK) from Hsp40 and decrease PKR phosphorylation PMID: 21698289
22. The antiviral activity of overexpressed DNAJC14 occurs in a time- and dose-dependent manner. PMID: 21249176
23. The crystal structure of full-length Hdj1 was diffracted to 3.90A resolution. PMID: 21139202
24. 5-fluorouracil & carboplatin specifically induce expression of Hsp40 in hepatoma cells. siRNA knockdown of Hsp40 diminishes survival of drug-exposed cells. PMID: 19901540
25. Altered expression of HSPF1 was found in brains and lymphoblastoid cells from patients with bipolar disorder. PMID: 14743183
26. sequestered in discrete foci in the nucleus of the infected cell in herpes simplex virus type 1 infection PMID: 15194794
27. It is confirmed an increased expression of HSPF1 in the lymphoblastoid cell lines from patients with bipolar I disorder, bipolar II disorder, and schizophrenia. PMID: 15362566
28. Hsp40 is crucial for Nef-mediated enhancement of viral gene expression and replication PMID: 16179353
29. hepatitis B virus X protein is the major target of Hdj1 in the inhibition of hepatitis B virus replication PMID: 16842747
30. These results demonstrate a role for Hsp40/DnaJB6 in the regulation of HIV-2 PIC nuclear transport. PMID: 18032501
31. The 2.7A structure reveals that Hdj1 forms a homodimer in the crystal by a crystallographic two-fold axis. PMID: 18211704
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亞細胞定位：

Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
數據庫鏈接：

HGNC: 5270

OMIM: 604572

KEGG: hsa:3337

STRING: 9606.ENSP00000254322

UniGene: Hs.515210

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